4.2.1.103: cyclohexyl-isocyanide hydratase

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For detailed information about cyclohexyl-isocyanide hydratase, go to the full flat file.

cyclohexyl isocyanide hydratase, ICH, InhB, isocyanide hydratase, isonitrile hydratase, More, N-cyclohexylformamide hydro-lyase

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Results	in table
737	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	Expression
4	General Information
37	Inhibitors
3	kcat/KM [mM/s]
6	KM Value [mM]
1	Metals/Ions
7	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
8	Organism
2	Pathway
16	PDB ID
3	pH Optimum
1	pH Range
1	pH Stability
20	Protein Variants
4	Purification (Commentary)
3	Reaction
1	Reaction Type
8	Reference
2	Source Tissue
6	Specific Activity [micromol/min/mg]
27	Substrates and Products (Substrate)
8	Subunits
18	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
3	Turnover Number [1/s]

Cloned on EC 4.2.1.103 - cyclohexyl-isocyanide hydratase

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expression of N-terminally His-tagged wild-type and mutant ICHs in Escherichia coli strain BL21(DE3)

gene inhA, DNA and amino acid sequence determination and analysis, overexpression of wild-type and mutant enzyme in Escherichia coli

InhB, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH10B