4.1.1.6: cis-aconitate decarboxylase

This is an abbreviated version!
For detailed information about cis-aconitate decarboxylase, go to the full flat file.

Reaction

Synonyms

Aconitate decarboxylase, CAD, CAD1, CadA, cis-aconitate decarboxylase, cis-Aconitic acid decarboxylase, Decarboxylase, aconitate

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.1 Carboxy-lyases

                4.1.1.6 cis-aconitate decarboxylase

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Results	in table
1	Activating Compound
23	AA Sequence
1	Application
1	CAS Registry Number
10	Cloned(Commentary)
10	Disease/ Diagnostics
5	General Information
1	General Stability
9	Inhibitors
1	KM Value [mM]
10	Localization
4	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
30	Organism
3	Pathway
2	PDB ID
4	pH Optimum
1	pH Range
3	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
28	Reference
1	Renatured (Commentary)
11	Source Tissue
1	Specific Activity [micromol/min/mg]
30	Substrates and Products (Substrate)
3	Subunits
47	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Cloned

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Cloned on EC 4.1.1.6 - cis-aconitate decarboxylase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

espressed as recombinant protein in Escherichia coli

Homo sapiens

-

708675

expressed in Corynebacterium glutamicum strain ATCC13032

Aspergillus terreus

-

748549

expressed in Escherichia coli BL21(DE3) cells

Aspergillus terreus

-

749341

expressed in Escherichia coli BL21(DE3) cells and in Aspergillus niger strain AB 1.13

Aspergillus terreus

-

715097

expressed in Escherichia coli BW25113 (DE3) cells

Aspergillus terreus

-

746886

expressed in Escherichia coli BW25113 (DE3) pLysS cells

Aspergillus terreus

-

747220

expressed in Saccharomyces cerevisiae

Aspergillus terreus

B3IUN8

690578

gene cadA, heterologous expression in Aspergillus niger strain N201, expression of two previously identified Aspergillus terreus genes encoding putative organic acid transporters, genes mttA and mfsA, increases itaconic acid production in an Aspergillus niger cis-aconitate decarboxylase expressing strain, production method optimization, overview

Aspergillus terreus

-

726774

gene cadA, heterologous functional expression in citric acid producing Aspergillus niger under various constitutive promoters of different expression strength, heterologous expression of the gene in Saccharomyces cerevisiae

Aspergillus terreus

-

727576

gene cadA, the cadA gene of is located in a gene cluster together with two transporters, one a putative mitochondrial carrier, the other a putative plasma membrane carrier, cloning in Escherichia coli strain TOP10, heterologous mitochondrial and cytosolic expression of the enzyme in Aspergillus niger strain ATCC 1015, the mitochondrial expression doubles the enzyme productivity compared to the cytosolic expression. Co-expression of a cytosolic or a mitochondrial aconitase with cytosolic CadA leads to a significant increase of itaconic acid production compared to cytsolic CadA expression alone. The rate-limiting step for itaconic acid production under these conditions appears to be both the provision of cis-aconitate as a substrate for the CadA as well as the CAD activity itself

Aspergillus terreus

-

728240