3.9.1.2: protein arginine phosphatase
This is an abbreviated version!
For detailed information about protein arginine phosphatase, go to the full flat file.
Reaction
Synonyms
YwlE
ECTree
Advanced search results
Application
Application on EC 3.9.1.2 - protein arginine phosphatase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
analysis
development of a substrate-trapping mutant that retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins. The substrate-trapping efficiency is improved by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captures arginine-phosphorylated proteins from complex Bacillus subtilis ywlE- cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications
analysis
-
development of a substrate-trapping mutant that retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins. The substrate-trapping efficiency is improved by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captures arginine-phosphorylated proteins from complex Bacillus subtilis ywlE- cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications
-