Application | Comment | Organism |
---|---|---|
analysis | development of a substrate-trapping mutant that retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins. The substrate-trapping efficiency is improved by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captures arginine-phosphorylated proteins from complex Bacillus subtilis ywlE- cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C9A | substrate-trapping mutant, retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39155 | - |
- |
Bacillus subtilis 168 | P39155 | - |
- |