3.5.4.31: S-methyl-5'-thioadenosine deaminase

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For detailed information about S-methyl-5'-thioadenosine deaminase, go to the full flat file.

Reaction

Synonyms

5-methylthioadenosine deaminase, 5-methylthioadenosine/S-adenosylhomocysteine deaminase, DadD, MJ1541, MTA deaminase, MtaD, MTADA, PA3170, Tm0936

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.4 In cyclic amidines

                3.5.4.31 S-methyl-5'-thioadenosine deaminase

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Results	in table
1	Activating Compound
6980	AA Sequence
4	Cloned(Commentary)
2	Crystallization (Commentary)
2	General Information
1	IC50 Value
7	Inhibitors
15	kcat/KM [mM/s]
6	Ki Value [mM]
15	KM Value [mM]
5	Metals/Ions
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
4	Organism
3	Pathway
2	PDB ID
1	pH Optimum
7	Protein Variants
3	Purification (Commentary)
1	Reaction
4	Reference
11	Substrates and Products (Substrate)
2	Subunits
14	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
4	Temperature Stability [°C]
15	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.5.4.31 - S-methyl-5'-thioadenosine deaminase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

50

Methanocaldococcus jannaschii

Q58936

10 min, mutant enzyme Y136R/E150R loses 65% of its activity, mutant enzyme Y136R loses 20% of its activity, mutant enzyme E150R loses% of its activity

727779

60

Methanocaldococcus jannaschii

Q58936

10 min, wild-type enzyme loses 10% of its activity, mutant enzyme Y136R loses 60% of its activity, mutant enzyme E150R loses 75% of its activity

727779

60 - 70

Methanocaldococcus jannaschii

Q58936

the enzyme shows 90% of retained activity after 10 min at 60°C and 34% of retained activity after 10 min at 70°C

727779

70

Methanocaldococcus jannaschii

Q58936

10 min, mutant enzyme loses 66% of its activity

727779