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3.5.1.77: N-carbamoyl-D-amino-acid hydrolase

This is an abbreviated version!
For detailed information about N-carbamoyl-D-amino-acid hydrolase, go to the full flat file.

Word Map on EC 3.5.1.77

Reaction

an N-carbamoyl-D-amino acid
+
H2O
=
a D-amino acid
+
NH3
+
CO2

Synonyms

AcHyuC, Amidase, N-carbamoyl-D-amino acid, BJS_06659, carbamoylase, D-carbamoylase, D-N-alpha-Carbamoylase, D-N-carbamoylase, D-NCAase, Dcase, DCase-M3, DCHase, hyuD, N-carbamoyl D-amino acid amidohydrolase, N-Carbamoyl-D-amino acid amidase, N-carbamoyl-D-amino acid amidohydrolase, N-Carbamyl amino acid amidohydrolase, N-Carbamyl-D-amino acid amidohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.77 N-carbamoyl-D-amino-acid hydrolase

General Stability

General Stability on EC 3.5.1.77 - N-carbamoyl-D-amino-acid hydrolase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high thermal stability and high stability in repeated batch reactions of the immobilized enzyme
-
immobilized enzyme is most stable at pH 7.0
-
immobilized enzyme is stabilized by dithiothreitol, L-Cys, cysteamine, and sodium hydrosulfite. After 14times repeated reactions, the remaining activity of the immobilized enzyme cross-linked with 0.1% and 0.2% of glutaraldehyde, and 0.2% of the glutaraldehyde with dithiothreitol in the reaction mixture is 12%, 18%, and 63% respectively
-
stable in absence rather than in presence of 2-mercaptoethanol
-