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3.5.1.70: aculeacin-A deacylase

This is an abbreviated version!
For detailed information about aculeacin-A deacylase, go to the full flat file.

Word Map on EC 3.5.1.70

Reaction

aculeacin A
+
H2O
=
palmitic acid
 +
deacyl-aculeacin A

Synonyms

AAC, aac1, aculeacin A acylase, AuAAC, cyclic lipopeptide acylase, deacylase, aculeacin A

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.70 aculeacin-A deacylase

Molecular Weight

Molecular Weight on EC 3.5.1.70 - aculeacin-A deacylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19000
-
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits
19100
-
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence
38000
-
HPLC gel filtration, the large discrepancy between the molecular weight estimated by HPLC and that estimated by SDS-PAGE is due to the particularly high pI of the enzyme: the molecular weight of acylase should be 74000 Da, the sum of the values of the two subunits obtained by SDS-PAGE
55000
-
1 * 55000 + 1 * 19000, SDS-PAGE or gel filtration in the presence of 6 M guanidine hydrochloride, two dissimilar subunits
60300
-
1 * 60300 + 1 * 19100, two dissimilar subunits, calculated from nucleotide sequence
84070
-
precursor peptide of aculeacin A acylase, calculated from nucleotide sequence
87000
-
precursor peptide of aculeacin A acylase, gel filtration and SDS-PAGE