3.4.24.78: gpr endopeptidase

This is an abbreviated version!
For detailed information about gpr endopeptidase, go to the full flat file.

Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala =

germinating-specific protease, GPS, germination proteinase, GPR, tepA, YmfB

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Results	in table
3493	AA Sequence
3	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
2	General Information
6	Inhibitors
6	KM Value [mM]
1	Metals/Ions
7	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
14	Organism
1	pH Optimum
1	pH Range
1	pH Stability
1	Posttranslational Modification
35	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
1	Specific Activity [micromol/min/mg]
28	Substrates and Products (Substrate)
4	Subunits
19	Synonyms
2	Temperature Stability [°C]

Cloned on EC 3.4.24.78 - gpr endopeptidase

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expression in Escherichia coli

expression of P41 in Escherichia coli

subcloned into the pET23a vector, overexpression of full-length gene P46 and a truncated form encoding only P41 in Escherichia coli Bl21(DE3)pLysS