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3.4.22.14: actinidain

This is an abbreviated version!
For detailed information about actinidain, go to the full flat file.

Word Map on EC 3.4.22.14

Reaction

Similar to that of papain =

Synonyms

Act d 1, ACT1A-1, ACT1A-2, Actd1, actinidain, actinidia anionic protease, actinidin, mercaptoproteinase A2, proteinase A2 of Actinidia chinensis

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.14 actinidain

Purification

Purification on EC 3.4.22.14 - actinidain

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Act d 1 is purified from kiwifruit extracts by covalent chromatography using activated thiopropyl sepharose 6B
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actinidin can be separated into six proteases, named KP1, KP2, KP3, KP4, KP5 and KP6
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Actinidin is purified from the soluble fraction of kiwi fruit by ion exchange chromatography on DE-52 and Mono-Q columns
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ammonium sulfate precipitation and DEAE-Sephadex gel filtration
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DEAE-Sephadex A-50 gel filtration and SP-Sephadex C-50 gel filtration
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multiple forms
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the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer