3.4.22.14: actinidain
This is an abbreviated version!
For detailed information about actinidain, go to the full flat file.
Word Map on EC 3.4.22.14
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3.4.22.14
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papain
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kiwifruit
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proteinases
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allergen
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kiwi
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deliciosa
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catalytic-site
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hayward
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ficin
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bromelain
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thaumatin-like
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chymopapains
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hort16a
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brocklehurst
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cysteine-proteinase
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kiwellin
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medicine
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two-protonic-state
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active-centre
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synthesis
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food industry
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nutrition
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molecular biology
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biotechnology
- 3.4.22.14
- papain
- kiwifruit
- proteinases
- allergen
-
kiwi
- deliciosa
-
catalytic-site
- hayward
- ficin
- bromelain
-
thaumatin-like
- chymopapains
-
hort16a
-
brocklehurst
-
cysteine-proteinase
- kiwellin
- medicine
-
two-protonic-state
-
active-centre
- synthesis
- food industry
- nutrition
- molecular biology
- biotechnology
Reaction
Similar to that of papain =
Synonyms
Act d 1, ACT1A-1, ACT1A-2, Actd1, actinidain, actinidia anionic protease, actinidin, mercaptoproteinase A2, proteinase A2 of Actinidia chinensis
ECTree
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Purification
Purification on EC 3.4.22.14 - actinidain
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Act d 1 is purified from kiwifruit extracts by covalent chromatography using activated thiopropyl sepharose 6B
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actinidin can be separated into six proteases, named KP1, KP2, KP3, KP4, KP5 and KP6
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Actinidin is purified from the soluble fraction of kiwi fruit by ion exchange chromatography on DE-52 and Mono-Q columns
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DEAE-Sephadex A-50 gel filtration and SP-Sephadex C-50 gel filtration
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the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer