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3.4.15.6: cyanophycinase

This is an abbreviated version!
For detailed information about cyanophycinase, go to the full flat file.

Word Map on EC 3.4.15.6

Reaction

[L-Asp(4-L-Arg)]n
+
H2O
=
[L-Asp(4-L-Arg)]n-1
 +
L-Asp(4-L-Arg)

Synonyms

CGPase, CphB, CphB1, CphB2, CphB3, CphB5, CphB6, CphE, CphEal, CphI, CphJ, cyanophycin granule polypeptidase, cyanophycinase, extracellular CGPase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.15 Peptidyl-dipeptidases
                3.4.15.6 cyanophycinase

Purification

Purification on EC 3.4.15.6 - cyanophycinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
centrifugation, proteins are concentrated and desalted using a cross-flow polyethersulfone cassette with a cutoff point of 30 kDa, a purification method for cyanophycinase is developed which depended on the strong affinity of the enzyme in the crude extract for its insoluble substrate cyanophycin
-
from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate