Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.15.6: cyanophycinase

This is an abbreviated version!
For detailed information about cyanophycinase, go to the full flat file.

Word Map on EC 3.4.15.6

Reaction

[L-Asp(4-L-Arg)]n
+
H2O
=
[L-Asp(4-L-Arg)]n-1
+
L-Asp(4-L-Arg)

Synonyms

CGPase, CphB, CphB1, CphB2, CphB3, CphB5, CphB6, CphE, CphEal, CphI, CphJ, cyanophycin granule polypeptidase, cyanophycinase, extracellular CGPase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.15 Peptidyl-dipeptidases
                3.4.15.6 cyanophycinase

Reference

Reference on EC 3.4.15.6 - cyanophycinase

Please use the Reference Search for a specific query.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Richter, R.; Hejazi, M.; Kraft, R.; Ziegler, K.; Lockau, W.
Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme
Eur. J. Biochem.
263
163-169
1999
Synechocystis sp. (P73832), Synechocystis sp.
Manually annotated by BRENDA team
Obst, M.; Oppermann-Sanio, F.B.; Luftmann, H.; Steinbüchel, A.
Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI
J. Biol. Chem.
277
25096-25105
2002
Pseudomonas anguilliseptica (Q8KQN8), Pseudomonas anguilliseptica, Pseudomonas anguilliseptica BI (Q8KQN8)
Manually annotated by BRENDA team
Fueser, G.; Steinbuechel, A.
Analysis of genome sequences for genes of cyanophycin metabolism: identifying putative cyanophycin metabolizing prokaryotes
Macromol. Biosci.
7
278-296
2007
Cupriavidus necator, Caulobacter vibrioides, Chloroflexus aurantiacus, Clostridium beijerinckii, Clostridium botulinum, Clostridium perfringens, Clostridium tetani, Acetivibrio thermocellus, Desulfitobacterium hafniense, Francisella tularensis, Mycobacterium marinum, Nostoc punctiforme, Nostoc sp., Rubrivivax gelatinosus, Synechococcus sp., Syntrophomonas wolfei, Thermoanaerobacter ethanolicus, Gloeobacter violaceus, Thermosynechococcus vestitus, Trichodesmium erythraeum, Chromohalobacter salexigens, Colwellia psychrerythraea, Acinetobacter baylyi, Crocosphaera watsonii, Gemmata obscuriglobus, Janibacter sp., Polaromonas sp., Trichormus variabilis (O86108), Pseudomonas anguilliseptica (Q8KQN8), Caldanaerobacter subterraneus subsp. tengcongensis (Q8R6L6), Chromohalobacter salexigens DSM 3043, Clostridium beijerinckii NCIMB 8052, Clostridium tetani E88, Syntrophomonas wolfei Goettingen, Chloroflexus aurantiacus J-10-fl, Pseudomonas anguilliseptica BI (Q8KQN8), Caulobacter vibrioides CB15, Crocosphaera watsonii WH 8501, Desulfitobacterium hafniense DCB-2, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Rubrivivax gelatinosus PM1
Manually annotated by BRENDA team
Sallam, A.; Kast, A.; Przybilla, S.; Meiswinkel, T.; Steinbüchel, A.
Biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization
Appl. Environ. Microbiol.
75
29-38
2009
Pseudomonas alcaligenes, Pseudomonas alcaligenes DIP1
Manually annotated by BRENDA team
Law, A.M.; Lai, S.W.; Tavares, J.; Kimber, M.S.
The structural basis of beta-peptide-specific cleavage by the serine protease cyanophycinase
J. Mol. Biol.
392
393-404
2009
Synechocystis sp. PCC 6803 (P73832)
Manually annotated by BRENDA team
Burnat, M.; Herrero, A.; Flores, E.
Compartmentalized cyanophycin metabolism in the diazotrophic filaments of a heterocyst-forming cyanobacterium
Proc. Natl. Acad. Sci. USA
111
3823-3828
2014
Nostoc sp., Nostoc sp. PCC 7120
Manually annotated by BRENDA team
Frommeyer, M.; Bergander, K.; Steinbuechel, A.
Guanidination of soluble lysine-rich cyanophycin yields a homoarginine-containing polyamide
Appl. Environ. Microbiol.
80
2381-2389
2014
Pseudomonas alcaligenes (G8HR04), Pseudomonas alcaligenes, Pseudomonas alcaligenes DIP1 (G8HR04)
Manually annotated by BRENDA team
Liu, H.; Ray, W.K.; Helm, R.F.; Popham, D.L.; Melville, S.B.
Analysis of the spore membrane proteome in Clostridium perfringens implicates cyanophycin in spore assembly
J. Bacteriol.
198
1773-1782
2016
Clostridium perfringens (Q0SQW9), Clostridium perfringens, Clostridium perfringens SM101 (Q0SQW9)
Manually annotated by BRENDA team
Nausch, H.; Broer, I.
Cyanophycinase CphE from P. alcaligenes produced in different compartments of N. benthamiana degrades high amounts of cyanophycin in plant extracts
Appl. Microbiol. Biotechnol.
101
2397-2413
2017
Pseudomonas alcaligenes (G8HR04), Pseudomonas alcaligenes
Manually annotated by BRENDA team
Ponndorf, D.; Ehmke, S.; Walliser, B.; Thoss, K.; Unger, C.; Goers, S.; Das, G.; Metges, C.C.; Broer, I.; Nausch, H.
Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine
Plant Biotechnol. J.
15
605-613
2017
Thermosynechococcus vestitus (P0C8P3)
Manually annotated by BRENDA team
Ponndorf, D.; Broer, I.; Nausch, H.
Expression of CphB- and CphE-type cyanophycinases in cyanophycin-producing tobacco and comparison of their ability to degrade cyanophycin in plant and plant extracts
Transgenic Res.
26
491-499
2017
Pseudomonas alcaligenes (G8HR04), Thermosynechococcus vestitus (P0C8P3)
Manually annotated by BRENDA team