3.3.2.15: trans-2,3-dihydro-3-hydroxyanthranilic acid synthase

This is an abbreviated version!
For detailed information about trans-2,3-dihydro-3-hydroxyanthranilic acid synthase, go to the full flat file.

Reaction

Synonyms

PhzD

ECTree

     3 Hydrolases

         3.3 Acting on ether bonds

             3.3.2 Ether hydrolases

                3.3.2.15 trans-2,3-dihydro-3-hydroxyanthranilic acid synthase

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Results	in table
10	AA Sequence
2	Cloned(Commentary)
1	Crystallization (Commentary)
1	Expression
4	General Information
3	kcat/KM [mM/s]
3	KM Value [mM]
4	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
5	Organism
5	Pathway
3	Protein Variants
1	Purification (Commentary)
3	Reaction
5	Reference
7	Substrates and Products (Substrate)
4	Subunits
2	Synonyms
1	Systematic Name
3	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.3.2.15 - trans-2,3-dihydro-3-hydroxyanthranilic acid synthase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate

4 entries

chorismate + H2O

?

Pseudomonas aeruginosa

Q7DC80

-

654709

-

-

?

isochorismate + H2O

2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

Pseudomonas aeruginosa

Q7DC80

hydrolyzed slightly less efficiently than (5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate (i.e. 2-amino-2-deoxy-isochorismate)

654709

-

-

?

additional information

?

-

Pseudomonas aeruginosa

Q7DC80

4-amino-4-deoxychorismate is barely hydrolyzed

654709

-

-

?

(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate

Pseudomonas aeruginosa

Q7DC80

i.e. 2-amino-2-deoxy-isochorismate. The enzyme is involved in phenazine biosynthesis

654709

i.e. trans-2,3-dihydro-3-hydroxyanthranilate

-

?

(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate

Pseudomonas aeruginosa

Q7DC80

i.e. 2-amino-2-deoxy-isochorismate. Slightly more efficiently hydrolyzed than isochorismate. Hydrolysis of the vinyl ether functional group. Slightly more efficiently than it hydrolyzes isochorismate

654709

i.e. trans-2,3-dihydro-3-hydroxyanthranilate

-

?

(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate

Pseudomonas fluorescens

Q51790

i.e. 2-amino-2-deoxy-isochorismate. The enzyme is involved in phenazine biosynthesis

704258

i.e. trans-2,3-dihydro-3-hydroxyanthranilate

-

?

(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate

Pseudomonas fluorescens Feb 79

Q51790

i.e. 2-amino-2-deoxy-isochorismate. The enzyme is involved in phenazine biosynthesis

704258

i.e. trans-2,3-dihydro-3-hydroxyanthranilate

-

?