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3.2.1.89: arabinogalactan endo-beta-1,4-galactanase

This is an abbreviated version!
For detailed information about arabinogalactan endo-beta-1,4-galactanase, go to the full flat file.

Word Map on EC 3.2.1.89

Reaction

Galbeta(1-6)Galbeta(1-4)(L-Arafalpha(1-3))Galbeta(1-4)Galbeta(1-4)(Galbeta(1-3)Galbeta(1-3)Galbeta
+
H2O
=
Galbeta(1-6)Galbeta
 +
L-Arafalpha(1-3)Galbeta(1-4)Galbeta(1-4)(Galbeta(1-3)Galbeta(1-3)Galbeta

Synonyms

arabinogalactanase, beta-1,4-galactanase, beta-D-galactanase, beta1,4-endogalactanase, BLGAL, E-EGAL, E-GAL, endo-1,4-beta-galactanase, endo-beta-(1->4)-D-galactanase, endo-beta-1,4-D-galactanase, endo-beta-1,4-galactanase, endo-beta-D-1,4-galactanase, endogalactanase A, GAL, GALA, galactanase, GALB, GH53 endo-beta-1,4-galactanase, More, PcGAL1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.89 arabinogalactan endo-beta-1,4-galactanase

Substrates Products

Substrates Products on EC 3.2.1.89 - arabinogalactan endo-beta-1,4-galactanase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aminopentyl-beta-D-galactopyranosyl-(1-> 4)-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranoside + H2O
?
show the reaction diagram
aminopentyl-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranoside + H2O
?
show the reaction diagram
aminopentyl-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranosyl-(1->4)-beta-D-galactopyranosyl-(1-> 4)-beta-D-galactopyranoside + H2O
?
show the reaction diagram
arabinogalactan + H2O
D-galactose
show the reaction diagram
-
initial release of D-galactotriose and D-galactotetraose, prolonged incubation results in D-galactose and D-galactobiose
-
-
?
arabinogalactan + H2O
galactose
show the reaction diagram
AZCL-galactan + H2O
?
show the reaction diagram
-
the enzyme shows a conserved beta-turn, characteristic of GH53 enzymes, which contributes to subsites -2 to +3
-
-
?
azurine cross-linked galactan + H2O
?
show the reaction diagram
-
enzyme releases blue color
-
-
?
azurine cross-linked lupin-galactan + H2O
?
show the reaction diagram
beta-(1->4)-D-galactotriose + H2O
D-galactose
show the reaction diagram
-
by galactanase I
-
-
?
beta-1,4-galactan
?
show the reaction diagram
-
main products are galactose and galactobiose
-
-
?
beta-1,4-galactan + H2O
?
show the reaction diagram
beta-1,4-galactan + H2O
galactobiose + galactotriose
show the reaction diagram
citrus fruit pectin + H2O
?
show the reaction diagram
debranched sugar beet arabinan + H2O
?
show the reaction diagram
galactan + H2O
?
show the reaction diagram
initially, rather long oligosaccharides are detected as hydrolysis products of potato galactan. Then, as the reaction proceeds, mainly galactotetraose is produced. After 24 h reaction, D-galactose, beta-1,4-D-galactobiose, beta-1,4-D-galactotriose and beta-1,4-D-galactotetraose are detected as the major products from potato galactan. The enzyme hydrolyzes galactans more efficiently than pectic galactans
-
-
?
galactan I + H2O
?
show the reaction diagram
-
isolated from black liquor, enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose
-
-
?
galactobiose + H2O
galactose
show the reaction diagram
-
by galactanase I
-
-
?
galactotetraose + H2O
galactose
show the reaction diagram
-
by galactanase I
-
-
?
lupin beta-1,4-galactan + H2O
?
show the reaction diagram
o-nitrophenyl-beta-D-galactopyranoside + H2O
o-nitrophenol + galactose + pyranose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + galactose
show the reaction diagram
-
-
-
-
?
pectic polysaccharide from Averrhoa carambola + H2O
?
show the reaction diagram
-
the substrate is composed of rhamnose, arabinose, galactose and uronic acid in the 5.0:72.5:12.1:10.4 molar ratios, respectively. The arabinan moiety is formed by (1->5)-linked alpha-L-Araf units in the backbone, branched at O-3 by (1->2)- and (1->3)-linked alpha-L-Araf units, while the type I arabinogalactan is formed by (1->4)- and(1->4,6)-linked beta-D-Galp units in the backbone with (1->5)-, (1-> 3,5)- and (1-> 3)-linked alpha-L-Araf units as side chains
-
-
?
potato galactan + H2O
galactotetraose
show the reaction diagram
soybean arabinogalactan + H2O
?
show the reaction diagram
sugarcane bagasse + H2O
?
show the reaction diagram
additional information
?
-