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Literature summary for 3.2.1.89 extracted from

  • Yang, H.; Ichinose, H.; Yoshida, M.; Nakajima, M.; Kobayashi, H.; Kaneko, S.
    Characterization of a thermostable endo-beta-1,4-D-galactanase from the hyperthermophile Thermotoga maritima (2006), Biosci. Biotechnol. Biochem., 70, 538-541.
    View publication on PubMed
Search for more literature for 3.2.1.89

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X0S8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactan + H2O initially, rather long oligosaccharides are detected as hydrolysis products of potato galactan. Then, as the reaction proceeds, mainly galactotetraose is produced. After 24 h reaction, D-galactose, beta-1,4-D-galactobiose, beta-1,4-D-galactotriose and beta-1,4-D-galactotetraose are detected as the major products from potato galactan. The enzyme hydrolyzes galactans more efficiently than pectic galactans Thermotoga maritima ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
endo-beta-1,4-D-galactanase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 50% of maximal activity, 100°C: about 95% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 pH 7.0, 60 min, no loss of activity Thermotoga maritima
85
-
 pH 7.0, 60 min, about 30% loss of activity Thermotoga maritima
90
-
 pH 7.0, 60 min, about 95% loss of activity Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 60°C, 60 min, about 75% loss of activity Thermotoga maritima
4 9 60°C, 60 min, less than 20% los of activity in the pH-range 4-9 Thermotoga maritima
10
-
 60°C, 60 min, about 60% of maximal activity Thermotoga maritima