3.2.1.181: galactan endo-beta-1,3-galactanase

This is an abbreviated version!
For detailed information about galactan endo-beta-1,3-galactanase, go to the full flat file.

Reaction

The enzyme specifically hydrolyses beta-1,3-galactan and beta-1,3-galactooligosaccharides =

Synonyms

Af3G, endo-beta-1,3-galactanase, FvEn3GAL, NcEn3GAL

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.181 galactan endo-beta-1,3-galactanase

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Results	in table
4	AA Sequence
4	Cloned(Commentary)
6	General Information
2	kcat/KM [mM/s]
3	KM Value [mM]
2	Molecular Weight [Da]
5	Organism
4	pH Optimum
2	pH Range
2	Posttranslational Modification
4	Protein Variants
4	Purification (Commentary)
3	Reaction
5	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
2	Subunits
8	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.2.1.181 - galactan endo-beta-1,3-galactanase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

arabinogalactan + H2O

?

3 entries

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

5 entries

beta-1,3-galactotetraose + H2O

2 beta-1,3-galactobiose

3 entries

beta-1,4-galactan + H2O

?

3 entries

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

2 entries

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

2 entries

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

2 entries

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + D-galactopyranose

Flammulina velutipes

F7J1C8

-

717837

after 30 min of reaction time the enzyme produces 42% D-glalactose, 30% dimeric, 20% trimeric and less than 10% tetrameric or pentameric beta-1,3-galactooligosaccharides

-

?

gum arabic beta-1,3-galactan I + H2O

?

3 entries

gum arabic beta-1,3-galactan II + H2O

?

3 entries

larch arabinogalactan + H2O

?

3 entries

larch beta-galactan I + H2O

?

3 entries

larch beta-galactan II + H2O

?

3 entries

larch beta-galactan III + H2O

?

3 entries

radish leaf arabinogalactan + H2O

?

2 entries

additional information

?

-

6 entries

arabinogalactan + H2O

?

Aspergillus flavus

-

the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme Af3G hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP

753156

-

-

?

arabinogalactan + H2O

?

Flammulina velutipes

-

from radish leaves

753156

-

-

?

arabinogalactan + H2O

?

Neurospora crassa

-

the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme NcEn3GAL hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP. Very low activity on native gum arabic and the larch arabinogalactan

753156

-

-

?

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

Aspergillus flavus

-

best substrate

753156

-

-

?

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

Flammulina velutipes

F7J1C8

-

717837

-

-

?

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

Flammulina velutipes

-

best substrate

753156

-

-

?

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

Flammulina velutipes NBRC 7663

F7J1C8

-

717837

-

-

?

beta-1,3-galactan + H2O

oligo-beta-(1->3)-D-galactose

Neurospora crassa

-

best substrate

753156

-

-

?

beta-1,3-galactotetraose + H2O

2 beta-1,3-galactobiose

Aspergillus flavus

-

the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose

753156

-

-

?

beta-1,3-galactotetraose + H2O

2 beta-1,3-galactobiose

Flammulina velutipes

-

-

753156

-

-

?

beta-1,3-galactotetraose + H2O

2 beta-1,3-galactobiose

Neurospora crassa

-

the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose

753156

-

-

?

beta-1,4-galactan + H2O

?

Aspergillus flavus

-

from lupin

753156

-

-

?

beta-1,4-galactan + H2O

?

Flammulina velutipes

-

from lupin

753156

-

-

?

beta-1,4-galactan + H2O

?

Neurospora crassa

-

from lupin

753156

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes

F7J1C8

-

717837

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes NBRC 7663

F7J1C8

-

717837

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes

F7J1C8

-

717837

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes NBRC 7663

F7J1C8

-

717837

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes

F7J1C8

-

717837

-

-

?

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O

3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

Flammulina velutipes NBRC 7663

F7J1C8

-

717837

-

-

?

gum arabic beta-1,3-galactan I + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

gum arabic beta-1,3-galactan I + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

gum arabic beta-1,3-galactan I + H2O

?

Neurospora crassa

-

-

753156

-

-

?

gum arabic beta-1,3-galactan II + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

gum arabic beta-1,3-galactan II + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

gum arabic beta-1,3-galactan II + H2O

?

Neurospora crassa

-

-

753156

-

-

?

larch arabinogalactan + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

larch arabinogalactan + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

larch arabinogalactan + H2O

?

Neurospora crassa

-

-

753156

-

-

?

larch beta-galactan I + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

larch beta-galactan I + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

larch beta-galactan I + H2O

?

Neurospora crassa

-

-

753156

-

-

?

larch beta-galactan II + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

larch beta-galactan II + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

larch beta-galactan II + H2O

?

Neurospora crassa

-

-

753156

-

-

?

larch beta-galactan III + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

larch beta-galactan III + H2O

?

Flammulina velutipes

-

-

753156

-

-

?

larch beta-galactan III + H2O

?

Neurospora crassa

-

-

753156

-

-

?

radish leaf arabinogalactan + H2O

?

Aspergillus flavus

-

-

753156

-

-

?

radish leaf arabinogalactan + H2O

?

Neurospora crassa

-

-

753156

-

-

?

additional information

?

-

Aspergillus flavus

-

very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview

753156

-

-

?

additional information

?

-

Flammulina velutipes

F7J1C8

The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity.

717837

-

-

?

additional information

?

-

Flammulina velutipes

-

The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity.

717837

-

-

?

additional information

?

-

Flammulina velutipes

-

substrate specificity, overview. Gum arabic from acacia tree is a poor substrate

753156

-

-

?

additional information

?

-

Flammulina velutipes NBRC 7663

F7J1C8

The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity.

717837

-

-

?

additional information

?

-

Neurospora crassa

-

very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview

753156

-

-

?