Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Flammulina velutipes |
Protein Variants | Comment | Organism |
---|---|---|
E138D | no activity | Flammulina velutipes |
E143D | no activity | Flammulina velutipes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
beta-1,3-galactan | the value for beta-1,3-galactan is 0.137 mg/ml | Flammulina velutipes | |
0.57 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes | |
1.25 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26690 | - |
calculated from amino acid sequence | Flammulina velutipes |
30000 | - |
SDS-PAGE | Flammulina velutipes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flammulina velutipes | F7J1C8 | winter mushroom | - |
Flammulina velutipes NBRC 7663 | F7J1C8 | winter mushroom | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Flammulina velutipes |
Purification (Comment) | Organism |
---|---|
- |
Flammulina velutipes |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
The enzyme specifically hydrolyses beta-1,3-galactan and beta-1,3-galactooligosaccharides | beta-1,4- and beta-1,6-galactooligosaccharides, agarose, or keratan sulfate do not serve as substrates | Flammulina velutipes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Flammulina velutipes | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.8 | - |
pH 5.0, 37°C, liberation of D-galactose | Flammulina velutipes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-galactan + H2O | - |
Flammulina velutipes | oligo-beta-(1->3)-D-galactose | - |
? | |
beta-1,3-galactan + H2O | - |
Flammulina velutipes NBRC 7663 | oligo-beta-(1->3)-D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes NBRC 7663 | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes NBRC 7663 | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes NBRC 7663 | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose | - |
? | |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O | - |
Flammulina velutipes | 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + D-galactopyranose | after 30 min of reaction time the enzyme produces 42% D-glalactose, 30% dimeric, 20% trimeric and less than 10% tetrameric or pentameric beta-1,3-galactooligosaccharides | ? | |
additional information | The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. | Flammulina velutipes | ? | - |
? | |
additional information | The enzyme does not hydrolyze beta1,3:1,4-glucan, beta-1,3:1,6-glucan, beta-1,3-glucan, xyloglucan, keratan sulfste, kappa-carrageenan, agaraose, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucuronide, 4-nitrophenyl beta-D-xyloside. It is inactive towards beta-1,4-galactan. It does not hydrolyze 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose. The enzyme requires at least 3 contiguous beta-1,3-residues. The enzyme shows a weak transglycosylation activity. | Flammulina velutipes NBRC 7663 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-galactanase | - |
Flammulina velutipes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Flammulina velutipes |
50 | - |
native enzyme | Flammulina velutipes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.47 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes | |
6.5 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Flammulina velutipes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes | |
4.38 | - |
beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose | pH 5.0, 37°C | Flammulina velutipes |