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3.2.1.156: oligosaccharide reducing-end xylanase

This is an abbreviated version!
For detailed information about oligosaccharide reducing-end xylanase, go to the full flat file.

Word Map on EC 3.2.1.156

Reaction

beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
+
H2O
= 2 beta-D-xylopyranosyl-(1-4)-alpha-D-xylopyranose

Synonyms

BaRex8A, BhRex8A, DFQ00_11062, GH8 xylanase, More, oligosaccharide reducing-end xylanase, PhXyl, reducing end xylose-releasing exo-oligoxylanase, reducing-end xylose-releasing exo-oligoxylanase, reducing-end xylose-releasing exooligoxylanase, Rex, Rex8A, RexA, rXyn8, XYLA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.156 oligosaccharide reducing-end xylanase

Purification

Purification on EC 3.2.1.156 - oligosaccharide reducing-end xylanase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
-
Halalkalibacterium halodurans
-
by using a Ni-NTA agarose column
Halalkalibacterium halodurans
-
by using a single-step HisTrap purification performed on a Atka fast protein liquid chromatograph. Protein yield: 140 mg recombinant enzyme per liter cell culture
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL-21 Codon-Plus(DE3) RIL by metal affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography to homogeneity