Application | Comment | Organism |
---|---|---|
biotechnology | a novel method for producing a glycosynthase from an inverting glycoside hydrolase by mutating a residue that holds the nucleophilic water molecule (Y198) with the general base residue while keeping the general base residue intact | Halalkalibacterium halodurans |
Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Halalkalibacterium halodurans |
Protein Variants | Comment | Organism |
---|---|---|
D263N | mutations at D263 results in decreased F- releasing activity | Halalkalibacterium halodurans |
Y198F | a mutation in the tyrosine residue changing it into phenylalanine causes a drastic decrease in the hydrolytic activity and a small increase in F- releasing activity from alpha-xylobiosyl fluoride in the presence of xylose. As a result of the high F- releasing activity and low hydrolytic activity, Y198F of Rex accumulates a large amount of product during the glycosynthase reaction. Km: 14.8 (xylose), kcat: 11.9/sec (xylose) | Halalkalibacterium halodurans |
Y198F/D263N | Y198F/D263N showed 20 times less F- releasing activity than Y198F, which is comparable to that of the corresponding single mutant, D263N. Km: 6.0 (xylose), kcat: 0.42/sec (xylose) | Halalkalibacterium halodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
D-xylose | mutant Y198F/D263N | Halalkalibacterium halodurans | |
6.6 | - |
D-xylose | - |
Halalkalibacterium halodurans | |
14.8 | - |
D-xylose | mutant Y198F | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using a Ni-NTA agarose column | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-xylobiosyl fluoride + D-xylose | - |
Halalkalibacterium halodurans | alpha-xylobiose + HF + D-xylose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rex | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Halalkalibacterium halodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
D-xylose | mutant Y198F/D263N | Halalkalibacterium halodurans | |
5.04 | - |
D-xylose | - |
Halalkalibacterium halodurans | |
11.9 | - |
D-xylose | mutant Y198F | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Halalkalibacterium halodurans |