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3.2.1.149: beta-primeverosidase

This is an abbreviated version!
For detailed information about beta-primeverosidase, go to the full flat file.

Word Map on EC 3.2.1.149

Reaction

a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside
+
H2O
=
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
 +
an alcohol

Synonyms

4-methylumbelliferyl-beta-D-glucopyranoside:beta-glucosidase, arbutinase, ary beta-glucosidase, beta-D-glucosidase, beta-D-glucoside glucohydrolase, beta-glucosidase, beta-glucoside hydrolase, beta-primeverosidase, diglycoside-specific beta-primeverosidase, p-nitrophenyl beta-glucosidase, primeverosidase, vicianase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.149 beta-primeverosidase

Substrates Products

Substrates Products on EC 3.2.1.149 - beta-primeverosidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-1-phenylethyl beta-D-glucopyranoside + H2O
(R)-1-phenylethanol + beta-D-glucopyranose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(R)-1-phenylethyl beta-primeveroside + H2O
(R)-1-phenylethanol + beta-primeverose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(R)-linalyl beta-vicianoside + H2O
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
(S)-1-phenylethyl beta-D-glucopyranoside + H2O
(S)-1-phenylethanol + beta-D-glucopyranose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(S)-1-phenylethyl beta-primeveroside + H2O
(S)-1-phenylethanol + beta-primeverose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(Z)-3-hexenyl beta-primeveroside + H2O
(Z)-3-hexenol + primeverose
show the reaction diagram
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.05% activity
-
-
?
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.60% activity
-
-
?
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
show the reaction diagram
-
trivial name beta-primeveroside
-
-
?
2-phenylethyl beta-acuminoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
i.e. 2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
-
?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + beta-D-glucopyranose
show the reaction diagram
low activity
-
-
?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + D-glucopyranose
show the reaction diagram
-
-
-
-
?
2-phenylethyl beta-gentiobioside + H2O
gentiobiose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.34% activity
-
-
?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + beta-primeverose
show the reaction diagram
-
-
-
?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + primeverose
show the reaction diagram
2-phenylethyl beta-primeveroside + H2O
primeverose + 2-phenylethanol
show the reaction diagram
2-phenylethyl beta-vicianoside + H2O
2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
2-phenylethyl beta-vicianoside + H2O
vicianose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 2.97% activity
-
-
?
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 3% of activity with 2-phenyl-beta-primeveroside
-
-
?
2-phenylethyl-6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 0.8% of activity with 2-phenyl-beta-primeveroside
-
-
?
2-phenylethyl-6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 0.25% of activity with 2-phenyl-beta-primeveroside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
pH 5.5, 40°C, 15% hydrolysis
-
-
?
4-nitrophenyl beta-primeveroside + H2O
4-nitrophenol + primeverose
show the reaction diagram
-
i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
?
4-nitrophenyl-beta-primeveroside + H2O
4-nitrophenol + beta-primeverose
show the reaction diagram
-
-
-
?
6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
alpha-L-arabinopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
beta-D-xylopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with highest activity
-
-
?
6-O-beta-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
beta-D-arabinopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
show the reaction diagram
a beta-primeveroside + H2O
? + primeverose
show the reaction diagram
-
the enzyme shows broad substrate specificity with respect to the disaccharide glycon moiety (subsite-2), Thr271 and Thr415 play important roles in subsite-2 of the enzyme
-
-
?
benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. 2-benzyl beta-D-primeveroside
-
?
benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose
show the reaction diagram
-
-
-
-
?
benzyl beta-primeveroside + H2O
phenylmethanol + primeverose
show the reaction diagram
cellobiose + H2O
2 D-glucose
show the reaction diagram
eugenyl beta-primeveroside + H2O
eugenol + primeverose
show the reaction diagram
furcatin + H2O
4-allyl-phenol + ?
show the reaction diagram
gentiobiose + H2O
D-glucose
show the reaction diagram
geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. geranyl beta-D-primeveroside
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
geranyl beta-primeveroside + H2O
geraniol + primeverose
show the reaction diagram
-
pH 5.5, 40°C, 0.1% hydrolysis
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
-
-
?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalool + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
-
-
?
p-nitrophenol beta-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
show the reaction diagram
pH 6.0, 37°C, 0.23% activity
-
-
?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
p-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
show the reaction diagram
-
trivial name beta-primeveroside
-
-
?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
primeverose + p-nitrophenol
show the reaction diagram
p-nitrophenyl beta-D-glucoside + H2O
p-nitrophenol + D-glucose
show the reaction diagram
pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme)
-
-
?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + D-gentiobiose
show the reaction diagram
-
pH 5.5, 40°C, 2.5% hydrolysis
-
-
?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + gentiobiose
show the reaction diagram
-
37°C, 41% hydrolysis
-
-
?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
show the reaction diagram
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
primeverose + H2O
D-xylose + D-glucose
show the reaction diagram
-
37°C, 0.2% hydrolysis
-
-
?
sophorose + H2O
2 D-glucose
show the reaction diagram
-
37°C, 0.5% hydrolysis
-
-
?
additional information
?
-