Information on EC 3.2.1.149 - beta-primeverosidase

Word Map on EC 3.2.1.149
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.149
-
RECOMMENDED NAME
GeneOntology No.
beta-primeverosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O = 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
show the reaction diagram
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides, i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides, it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tea aroma glycosidic precursor bioactivation
-
-
SYSTEMATIC NAME
IUBMB Comments
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside 6-O-(beta-D-xylosyl)-beta-D-glucohydrolase
The enzyme is responsible for the formation of the alcoholic aroma in oolong and black tea. In addition to beta-primeverosides [i.e. 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranosides], it also hydrolyses 6-O-(beta-D-apiofuranosyl)-beta-D-glucopyranosides and, less rapidly, beta-vicianosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides, but not beta-glucosides. Geranyl-, linaloyl-, benzyl- and p-nitrophenol glycosides are all hydrolysed.
CAS REGISTRY NUMBER
COMMENTARY hide
884593-92-4
-
9001-22-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AP-20
-
-
Manually annotated by BRENDA team
IAM7153
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
beta-primeverosidase is a disaccharide-specific beta-glycosidase in tea leaves. This enzyme is involved in aroma formation during the manufacturing process of oolong tea and black tea. The enzyme hydrolyzes beta-primeveroside at the beta-glycosidic bond of primeverose to aglycone, and releases aromatic alcoholic volatiles of aglycones
additional information
-
three-dimensional structures of the enzyme in apo and complex forms with N-beta-primeverosylamidines, overview. Three subsites in the active site: subsite -2 specific for 6-O-beta-D-xylopyranosyl, subsite -1 well conserved among beta-glucosidases and specific for beta-D-glucopyranosyl, and wide subsite +1 for hydrophobic aglycone. Glu470, Ser473, and Gln477 act as the specific hydrogen bond donors for 6-O-beta-D-xylopyranosyl in subsite +2. On the other hand, subsite +1 is a large hydrophobic cavity that accommodates various aromatic aglycones. Tight binding of the disaccharide in the deep active site, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-1-phenylethyl beta-D-glucopyranoside + H2O
(R)-1-phenylethanol + beta-D-glucopyranose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(R)-1-phenylethyl beta-primeveroside + H2O
(R)-1-phenylethanol + beta-primeverose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(R)-linalyl beta-vicianoside + H2O
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
(S)-1-phenylethyl beta-D-glucopyranoside + H2O
(S)-1-phenylethanol + beta-D-glucopyranose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(S)-1-phenylethyl beta-primeveroside + H2O
(S)-1-phenylethanol + beta-primeverose
show the reaction diagram
LC-MS analysis and quantitative GC-MS analysis of substrate and product
-
-
?
(Z)-3-hexenyl beta-primeveroside + H2O
(Z)-3-hexenol + primeverose
show the reaction diagram
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.05% activity
-
-
?
2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.60% activity
-
-
?
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
show the reaction diagram
-
trivial name beta-primeveroside
-
-
?
2-phenylethyl beta-acuminoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
i.e. 2-phenylethyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
-
?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + beta-D-glucopyranose
show the reaction diagram
-
low activity
-
-
?
2-phenylethyl beta-D-glucopyranoside + H2O
2-phenylethanol + D-glucopyranose
show the reaction diagram
-
-
-
-
?
2-phenylethyl beta-gentiobioside + H2O
gentiobiose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 0.34% activity
-
-
?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + beta-primeverose
show the reaction diagram
-
-
-
-
?
2-phenylethyl beta-primeveroside + H2O
2-phenylethanol + primeverose
show the reaction diagram
2-phenylethyl beta-primeveroside + H2O
primeverose + 2-phenylethanol
show the reaction diagram
2-phenylethyl beta-vicianoside + H2O
2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
2-phenylethyl beta-vicianoside + H2O
vicianose + 2-phenylethanol
show the reaction diagram
pH 6.0, 37°C, 2.97% activity
-
-
?
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 3% of activity with 2-phenyl-beta-primeveroside
-
-
?
2-phenylethyl-6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 0.8% of activity with 2-phenyl-beta-primeveroside
-
-
?
2-phenylethyl-6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-glucopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
trivial name beta-vicianoside, 0.25% of activity with 2-phenyl-beta-primeveroside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
pH 5.5, 40°C, 15% hydrolysis
-
-
?
4-nitrophenyl beta-primeveroside + H2O
4-nitrophenol + primeverose
show the reaction diagram
-
i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
?
6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
alpha-L-arabinopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
beta-D-apiofuranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
beta-D-xylopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with highest activity
-
-
?
6-O-beta-L-arabinopyranosyl-beta-D-glucopyranoside + H2O
beta-D-arabinopyranose + beta-D-glucopyranose
show the reaction diagram
hydrolyzed with lower activity
-
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
show the reaction diagram
a beta-primeveroside + H2O
? + primeverose
show the reaction diagram
-
the enzyme shows broad substrate specificity with respect to the disaccharide glycon moiety (subsite-2), Thr271 and Thr415 play important roles in subsite-2 of the enzyme
-
-
?
benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. 2-benzyl beta-D-primeveroside
-
?
benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose
show the reaction diagram
-
-
-
-
?
benzyl beta-primeveroside + H2O
phenylmethanol + primeverose
show the reaction diagram
cellobiose + H2O
D-glucose
show the reaction diagram
eugenyl beta-primeveroside + H2O
eugenol + primeverose
show the reaction diagram
furcatin + H2O
4-allyl-phenol + ?
show the reaction diagram
gentiobiose + H2O
D-glucose
show the reaction diagram
geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. geranyl beta-D-primeveroside
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
geranyl beta-primeveroside + H2O
geraniol + primeverose
show the reaction diagram
-
pH 5.5, 40°C, 0.1% hydrolysis
-
-
?
laminaribiose + H2O
D-glucose
show the reaction diagram
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
-
-
?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalool + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
-
-
?
p-nitrophenol beta-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
show the reaction diagram
pH 6.0, 37°C, 0.23% activity
-
-
?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
p-nitrophenol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
show the reaction diagram
-
trivial name beta-primeveroside
-
-
?
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
primeverose + p-nitrophenol
show the reaction diagram
p-nitrophenyl beta-D-glucoside + H2O
p-nitrophenol + D-glucose
show the reaction diagram
pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme)
-
-
?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + D-gentiobiose
show the reaction diagram
-
pH 5.5, 40°C, 2.5% hydrolysis
-
-
?
p-nitrophenyl beta-gentiobioside + H2O
p-nitrophenol + gentiobiose
show the reaction diagram
-
37°C, 41% hydrolysis
-
-
?
p-nitrophenyl beta-primeveroside + H2O
primeverose + p-nitrophenol
show the reaction diagram
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
-
?
primeverose + H2O
D-xylose + D-glucose
show the reaction diagram
-
37°C, 0.2% hydrolysis
-
-
?
sophorose + H2O
D-glucose
show the reaction diagram
-
37°C, 0.5% hydrolysis
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-linalyl beta-vicianoside + H2O
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
2-phenylethyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O
2-phenylethanol + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
show the reaction diagram
-
trivial name beta-primeveroside
-
-
?
2-phenylethyl beta-vicianoside + H2O
2-phenylethanol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
a 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
show the reaction diagram
-
-
-
-
?
benzyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
benzyl alcohol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. 2-benzyl beta-D-primeveroside
-
?
eugenyl beta-primeveroside + H2O
eugenol + primeverose
show the reaction diagram
-
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
-
-
-
geranyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
geraniol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
i.e. geranyl beta-D-primeveroside
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
linalyl 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H2O
linalol + 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-phenyl-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)ethylamidine
-
enzyme binding structure, overview
4-(N-benzylsuccinimide-3-sulfanyl)-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)butylamidine
-
enzyme binding structure, overview
N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
strongly inhibits
N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
potent inhibitor
additional information
-
not inhibited by beta-glucosylamidine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
2
2-phenylethyl beta-primeveroside
pH 6.0, 37°C
2 - 14.9
2-phenylethyl beta-vicianoside
14.9
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
-
37°C, pH 6.0
2.8 - 6.3
eugenyl beta-primeveroside
2.9
p-nitrophenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
-
37°C
47
p-nitrophenyl beta-D-glucopyranoside
-
pH 5.5, 40°C
6.3 - 21
p-nitrophenyl beta-gentiobioside
12
p-nitrophenyl beta-primeveroside
-
pH 5.5, 40°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
43.7
2-phenylethyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside
Camellia sinensis
-
37°C, pH 6.0
43.8
2-phenylethyl beta-primeveroside
Camellia sinensis
Q7X9A9
pH 6.0, 37°C
5.83 - 43.7
2-phenylethyl beta-vicianoside
5.85
2-phenylethyl-6-O-alpha-D-arabinosyl-beta-D-glucopyranoside
Camellia sinensis
-
37°C, pH 6.0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
4-(N-benzylsuccinimide-3-sulfanyl)-N-(6-O-beta-D-xylopyranosyl-beta-D-glucopyranosyl)butylamidine
-
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.14
N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
-
0.026
N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
glucosidase I
0.15
-
glucosidase II
0.27
-
glucosidase IIa
0.44
-
glucosidase IIb
0.98
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
0.99
measured with p-nitrophenol beta-primeveroside in 20 mM citrate buffer (pH 6.0) at 37°C
1.5
-
purified enzyme
1.61
-
glucosidase III
1.7
purified enzyme
4.4
-
purified enzyme
26.8
-
30fold purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 6
-
less than 50% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
var. sinensis cv. Yabukita
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
-
less than 50% of maximal activity above and below
50 - 60
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low expression level
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
and in the cavity area among cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
x * 47000, SDS-PAGE
50000
-
monomer, SDS-PAGE, gel filtration
60000
-
1 * 60000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
deglycosylation by alpha-mannosidase
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant alpha-mannosidase-treated enzyme, in complex with disaccharide amidine inhibitors, sitting drop vapor diffusion method, mixing of 0.0015 ml of 10 mg/ml protein in 10mM sodium acetate, with 0.0015 ml of reservoir solution containing 0.01% Triton X-100, 21% PEG 4000, 0.3 M ammonium acetate, and 0.1 M sodium citrate, pH 5.5, 20°C, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution, molecular replacement
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 5
-
666247
3 - 8
-
-
288805
4 - 5
-
666247
4 - 9
-
-
288804
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
estimated from the residual activities after incubation in 20 mM citrate buffer (pH 6.0) at various temperatures (30-70°C)
55
-
1 h, 50% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration and affinity chromatography using a primeverosylamidine column, 30fold purified
-
recombinant enzyme from insect cells by cation exchange chromatography and ConA affinity chromatography with N-beta-primeverosylamidine adsorbent
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and desalting gel filtration
var. assamica
-
var. sinensis cv. Shuixian
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; expression in Escherichia coli
cloned from tea flowers, recombinant inducible expression of His-tagged enzyme in Escherichia coli
expression in Escherichia coli
full-length cDNA, including a putative N-terminal secretion signal sequence, cloned into the baculovirus expression plasmid, expressed in Sf9 cells or High-five cells
-
sequence comparison, the enzyme including the N-terminal signal sequence is cloned and expressed in a baculovirus-insect cell system
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
beta-primeverosidase is a key enzyme in tea aroma formation