3.2.1.145: galactan 1,3-beta-galactosidase

This is an abbreviated version!
For detailed information about galactan 1,3-beta-galactosidase, go to the full flat file.

Reaction

Synonyms

arabinogalactan endo-1,3-beta-galactosidase, BbGal43A, beta-galactanase, Bl1,3Gal, Bl1,6Gal, BLLJ_1840, Ct1,3Gal43A, Cthe_0661, exo-/beta-1,3-galactanase, Exo-1,3-Gal, exo-beta-(1,3)-D-galactanase, exo-beta-(1->3)-D-galactanase, exo-beta-1,3-D -galactanase, exo-beta-1,3-D-galactanase, exo-beta-1,3-galactanase, exo-beta-1,6-galactobiohydrolase, Fo/1,3Gal, GH30_5 exo-beta-1,6-galactobiohydrolase, GH43_24 exo-beta-1,3-galactanase, Il1,3Gal, More, Pc1,3Gal43A, RsBGAL1, Sa1,3Gal43A, SGalase1, SGalase2

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.145 galactan 1,3-beta-galactosidase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
16	AA Sequence
2	Application
2	CAS Registry Number
12	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
18	General Information
4	Inhibitors
6	KM Value [mM]
15	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
24	Organism
10	PDB ID
13	pH Optimum
3	pH Range
2	pH Stability
1	pI Value
2	Posttranslational Modification
4	Protein Variants
12	Purification (Commentary)
1	Reaction
1	Reaction Type
24	Reference
1	Source Tissue
25	Specific Activity [micromol/min/mg]
90	Substrates and Products (Substrate)
22	Subunits
52	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 3.2.1.145 - galactan 1,3-beta-galactosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography

Sphingomonas sp.

-

713904

DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase

Irpex lacteus

-

171335

native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Streptomyces sp.

I0B0S9, I0B0T0

731581

Ni-nitrilotriacetic acid agarose colum

Acetivibrio thermocellus

A3DD67

677662

Ni-NTA agarose column

Streptomyces avermitilis

-

678786

recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)

Bifidobacterium longum subsp. Longum

A0A401ETL2

752571

recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration

Irpex lacteus

-

707784

recombinant enzyme expressed in Pichia pastoris

Raphanus sativus

Q6L619

666634

recombinant expression of His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Acetivibrio thermocellus

A3DD67

732396

recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography

Phanerodontia chrysosporium

-

706965

recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography

Fusarium oxysporum

B6F260

731202

recombinant Trx-His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, tag cleavage through TEV protease, and another step of nickel affinity chromatography, followed by gel filtration, to homogeneity

Bifidobacterium bifidum

E4VCC5

752397