Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Acetivibrio thermocellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DD67 | precursor | - |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetic acid agarose colum | Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.3 | - |
beta-1,3-galactan as a substrate | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-arabinofuranosidase-treated root arabinogalactan proteins from radish + H2O | relative activity: 9.3 | Acetivibrio thermocellus | ? | - |
? | |
beta-(1-3)-D-galactotriose + H2O | relative activity: 119%. Enzyme hydrolyzes beta-1,3-linked galacto-oligosaccharides but does not hydrolyze beta-1,4-linked and beta-1,6-linked galacto-oligosaccharide. Ct1,3Gal43A also can not hydrolyze the beta-1,3-galactosyl linkage of beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl L-arabinofuranoside | Acetivibrio thermocellus | beta-(1-3)-D-galactobiose + D-galactose | - |
? | |
beta-(1-3)-galactobiose + H2O | relative activity: 31% | Acetivibrio thermocellus | D-galactose | - |
? | |
beta-1,3-beta-1,6-galactan + H2O | relative activity: 3.1% | Acetivibrio thermocellus | ? | - |
? | |
beta-1,3-galactan + H2O | relative activity: 100%. Enzyme catalyzes hydrolysis only of beta-1,3-linked galactosyl oligosaccharides and polysaccharides. Enzyme does not hydrolyze beta-1,4-linked galactose-containing polysaccharides, such as pectic galactan and alpha-L-arabinofuranosidase-treated pectic galactan from lupine, and also does not hydrolyze laminarin, CM-curdlan, or beta-1,3-xylan, suggesting that the enzyme acts only on a beta-1,3-linked galactan chain | Acetivibrio thermocellus | oligo-beta-(1->3)-D-galactose + D-galactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ct1,3Gal43A | - |
Acetivibrio thermocellus |
exo-beta-1,3-D-galactanase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Acetivibrio thermocellus |
50 | - |
- |
Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | - |
Acetivibrio thermocellus |