Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.99.B3: Hef endonuclease

This is an abbreviated version!
For detailed information about Hef endonuclease, go to the full flat file.

Reaction

structure-specific endonuclease cleaving the DNA strand at the 5'-side of nicked or flapped positions in the duplex DNA. =

Synonyms

HEF, Hef nuclease, HefHhH, helicase-associated endonuclease for fork-structured DNA

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
            
                3.1.99.B3 Hef endonuclease

Advanced search results

Do not include text mining results
Include results (more...)
Include results (more...)
Resultsin table
1Cloned(Commentary)
1Crystallization (Commentary)
3General Information
3Organism
1Purification (Commentary)
1Reaction
3Reference
2Substrates and Products (Substrate)
5Synonyms

Purification

Purification on EC 3.1.99.B3 - Hef endonuclease

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length Hef protein can not be produced in Escherichia coli. The gene for the N-terminal domain (Met1-Ala546) is easily cloned into the same vector. The gene product is designated as N546. The cloning of the gene for the C-terminal domain (Gln547-Glu763) into the same expression vector is difficult. One clone is obtained, however, the plasmid from the clone contains a single base substitution, which causes a single base substitution, which causes a single amino acid change from Phe to Leu. The mutant protein has the same cleavage specificity and is designated C547'
-