Information on EC 3.1.99.B3 - Hef endonuclease

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.99.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Hef endonuclease
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
structure-specific endonuclease cleaving the DNA strand at the 5'-side of nicked or flapped positions in the duplex DNA.
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
deletion of hef confers only moderate sensitivity to DNA crosslinking agents
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
full-length Hef protein can not be produced in Escherichia coli. The gene for the N-terminal domain (Met1-Ala546) is easily cloned into the same vector. The gene product is designated as N546. The cloning of the gene for the C-terminal domain (Gln547-Glu763) into the same expression vector is difficult. One clone is obtained, however, the plasmid from the clone contains a single base substitution, which causes a single base substitution, which causes a single amino acid change from Phe to Leu. The mutant protein has the same cleavage specificity and is designated C547'
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE