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3.1.11.3: exodeoxyribonuclease (lambda-induced)

This is an abbreviated version!
For detailed information about exodeoxyribonuclease (lambda-induced), go to the full flat file.

Word Map on EC 3.1.11.3

Reaction

exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates =

Synonyms

5'exonuclease, EC 3.1.4.28, exonuclease, lambda exo, lambda exonuclease, lambda-exo, lambda-exonuclease, phage lambda-induced exonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.3 exodeoxyribonuclease (lambda-induced)

Engineering

Engineering on EC 3.1.11.3 - exodeoxyribonuclease (lambda-induced)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D119A
Lambdavirus lambda
-
site-directed mutagenesis, inactive mutant
E85A
Lambdavirus lambda
-
site-directed mutagenesis, almost inactive mutant
K131A
Lambdavirus lambda
-
site-directed mutagenesis, inactive mutant
K49A
Lambdavirus lambda
-
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
K76A
Lambdavirus lambda
-
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
L78A
Lambdavirus lambda
-
site-directed mutagenesis, the mutant shows a significantly diminished level of activity compared to the wild-type enzyme
M53A
Lambdavirus lambda
-
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme
R137A
Lambdavirus lambda
-
site-directed mutagenesis, inactive mutant
R28A
Lambdavirus lambda
-
site-directed mutagenesis, mutation of a residue that contact the 5' phosphate of the DNA, inactive mutant
R45A
Lambdavirus lambda
-
site-directed mutagenesis, inactive mutant
W24A
Lambdavirus lambda
-
site-directed mutagenesis, mutation of a residue that contact the 5' phosphate of the DNA, almost inactive mutant