Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.11.3: exodeoxyribonuclease (lambda-induced)

This is an abbreviated version!
For detailed information about exodeoxyribonuclease (lambda-induced), go to the full flat file.

Word Map on EC 3.1.11.3

Reaction

exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates =

Synonyms

5'exonuclease, EC 3.1.4.28, exonuclease, lambda exo, lambda exonuclease, lambda-exo, lambda-exonuclease, phage lambda-induced exonuclease

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.3 exodeoxyribonuclease (lambda-induced)

Application

Application on EC 3.1.11.3 - exodeoxyribonuclease (lambda-induced)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
molecular biology
Lambdavirus lambda
nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (lambda-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent strands intact. LexoG0 data to control for nascent strand-independent lambda-exo biases in NSseq and validated this approach at the rDNA locus. Possible increase of both sensitivity and specificity of NS-seq, overview
synthesis
Lambdavirus lambda
-
solid-phase digestion of dsDNAs using immobilization of lambda exonuclease onto poly(methylmethacrylate) micropillars populated within a microfluidic device for the on-chip digestion. The efficiency for the catalysis of dsDNA digestion using lamba-exonuclease, including its processivity and reaction rate, are higher when the enzyme is attached to a solid support compared to the free solution digestion. A clipping rate of 1000 nucleotides per s can be obtained for the digestion of lambda-DNA (48.5 kbp) by lambda-exonuclease
additional information
-
preparation of single-stranded DNA is an essential and important step in the combinatorial chemistry technique SELEX (Systematic Evolution of Ligands by EXponential enrichment) for in vitro selection of single-stranded DNA aptamers and numerous other molecular biology procedures, whereby single-stranded DNA generation by lambda exonuclease digestion is superior to other techniques. Important role for complete lambda exonuclease digestion of phosphorylated DNA strand plays the manufacturing of phosphorylated primer