2.1.1.337: reticuline N-methyltransferase

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For detailed information about reticuline N-methyltransferase, go to the full flat file.

Reaction

Synonyms

NMT3, RNMT

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.337 reticuline N-methyltransferase

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
1	Cofactor
1	General Information
3	kcat/KM [mM/s]
11	KM Value [mM]
2	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
2	Organism
3	Pathway
2	pH Optimum
1	pI Value
2	Protein Variants
2	Reaction
2	Reference
1	Source Tissue
14	Substrates and Products (Substrate)
1	Subunits
4	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
11	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.1.337 - reticuline N-methyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E204G

Papaver somniferum

A0A1C9U5X5

mutation to the corresponding residue of Coptis japonica coclaurine N-methyltransferase. The mutation has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat

757949

G204E

Papaver somniferum

A0A1C9U5X5

the mutation results in an enzyme with reduced canonical reticuline N-methyltransferase activity and increased canonical coclaurine N-methyltransferase activity. The mutant enzyme shows activity with (S)-coclaurine, which is modestly better than with (S)-N-methylcoclaurine but worse than with (S)-reticuline. A G204E substitution has no appreciable effect on Km for (S)-coclaurine but increases Km for (S)-N-methylcoclaurine approximately 4fold. kcat for (S)-coclaurine is 10fold greater while that for (S)-N-methylcoclaurine decreases 4fold. The G204E substitution has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat

757949