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1.14.13.218: 5-methylphenazine-1-carboxylate 1-monooxygenase

This is an abbreviated version!
For detailed information about 5-methylphenazine-1-carboxylate 1-monooxygenase, go to the full flat file.

Reaction

5-methylphenazine-1-carboxylate
+
NADH
+
O2
=
pyocyanin
+
NAD+
+
CO2
+
H2O

Synonyms

PA4217, phzS

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.218 5-methylphenazine-1-carboxylate 1-monooxygenase

Crystallization

Crystallization on EC 1.14.13.218 - 5-methylphenazine-1-carboxylate 1-monooxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using 0.7-1.0 M lithium sulfate, 0.1 M sodium citrate pH 5.0–5.5, 0.5 M ammonium sulfate or 14-17% (w/v) PEG 10 000, 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5 and 23-26% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.8-6.0, 0.2 M ammonium sulfate
-