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Literature summary for 1.14.13.218 extracted from

  • Gohain, N.; Thomashow, L.S.; Mavrodi, D.V.; Blankenfeldt, W.
    The purification, crystallization and preliminary structural characterization of FAD-dependent monooxygenase PhzS, a phenazine-modifying enzyme from Pseudomonas aeruginosa (2006), Acta Crystallogr. Sect. F, 62, 989-992.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta pLysS cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
native and seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using 0.7-1.0 M lithium sulfate, 0.1 M sodium citrate pH 5.0–5.5, 0.5 M ammonium sulfate or 14-17% (w/v) PEG 10 000, 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5 and 23-26% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.8-6.0, 0.2 M ammonium sulfate Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43600
-
-
Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-methylphenazine-1-carboxylate + NADH + O2 Pseudomonas aeruginosa
-
pyocyanin + NAD+ + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity resin column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methylphenazine-1-carboxylate + NADH + O2
-
Pseudomonas aeruginosa pyocyanin + NAD+ + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 43600, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
phzS
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas aeruginosa
NADH
-
Pseudomonas aeruginosa