1.14.13.167: 4-nitrophenol 4-monooxygenase
This is an abbreviated version!
For detailed information about 4-nitrophenol 4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.167
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1.14.13.167
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sumo
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ubiquitin-conjugating
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sumoylation
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hydroxyquinol
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sumo-conjugating
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two-component
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rhodococcus
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ubiquitin-like
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para-nitrophenol
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hydroquinone
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1,2-dioxygenase
- 1.14.13.167
-
sumo
-
ubiquitin-conjugating
-
sumoylation
- hydroxyquinol
-
sumo-conjugating
-
two-component
-
rhodococcus
-
ubiquitin-like
- para-nitrophenol
- hydroquinone
-
1,2-dioxygenase
Reaction
Synonyms
2C4NP 4-monooxygenase, 4-NP/4-NCA monooxygenase, NpcA, para-nitrophenol 4-monooxygenase, PdcA, PNP 4-monooxygenase, pnpA, PnpA1
ECTree
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Crystallization
Crystallization on EC 1.14.13.167 - 4-nitrophenol 4-monooxygenase
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sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol
structure in its apo and FAD-complex to a resolution of 2.04 A and 2.48 A, respectively. The 4-nitrophenol substrate lies near the N5 edge of isoalloxazine ring in the protein interior. The bound substrate is rotated 60° with respect to pOHB deviating from the isoalloxazine. The nitro group of 4-nitrophenol is directly hydrogen bonded to Val54 and Arg234, and the hydroxyl group is hydrogen bonded to Val223 and Cys23