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1.14.13.167: 4-nitrophenol 4-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.167

Reaction

4-nitrophenol
+
NADPH
+
H+
+
O2
=
1,4-benzoquinone
+
nitrite
+
NADP+
+
H2O

Synonyms

2C4NP 4-monooxygenase, 4-NP/4-NCA monooxygenase, NpcA, para-nitrophenol 4-monooxygenase, PdcA, PNP 4-monooxygenase, pnpA, PnpA1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.167 4-nitrophenol 4-monooxygenase

Purification

Purification on EC 1.14.13.167 - 4-nitrophenol 4-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
-
Ni-NTA agarose column chromatography and Sephadex G-100 gel filtration
Ni-NTA column chromatography
recombinant N-terminally His6-tagged enzyme PnpA from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography