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1.11.1.11: L-ascorbate peroxidase

This is an abbreviated version!
For detailed information about L-ascorbate peroxidase, go to the full flat file.

Word Map on EC 1.11.1.11

Reaction

2 L-ascorbate +

H2O2
+ 2 H+ = 2 monodehydroascorbate + 2 H2O

Synonyms

Am-pAPX1, APEX2, APOX, APX, APX 1, APX 2, APX1, APX2, APX3, APX4, APX5, APX6, APX7, APx8, APXS, APXT, ascorbate peroxidase, ascorbate peroxidase 1, ascorbate peroxidase 2, ascorbate peroxidase 3, ascorbate peroxidase 4, ascorbate peroxidase 5, ascorbate peroxidase 6, ascorbate peroxidase 7, ascorbate peroxidase 8, ascorbate peroxidase6, ascorbic acid peroxidase, At1g07890, AT1G77490, AT3G09640, AT4G08390, AT4G32320, AT4G35000, AT4G35970, AtAPx08, AtAPX1, AtAPX2, AtAPX3, AtAPX5, AtAPX6, AtSAPX, AtstAPX, AtTAPX, cAPX, cAPX 2, CmstAPX, CrAPX4, CreAPX1, CreAPX2, CreAPX4, CreAPXheme, CsAPX1, cytoplasmic ascorbate peroxidase 1, cytosolic ascorbate peroxidase, GhAPX1, glyoxysomal APX, HvAPX1, L-ascorbate peroxidase, L-ascorbate peroxidase 3, L-ascorbate peroxidase 5, L-ascorbate peroxidase 6, L-ascorbate peroxidase, heme-containing, L-ascorbic acid peroxidase, L-ascorbic acid-specific peroxidase, LmAPX, MaAPX1, OsAPx1, OsAPx2, OsAPx3, OsAPx4, OsAPx5, OsAPx6, OsAPx7, OsAPx8, OsAPXa, OsAPXb, pAPX, Pavirv00022559m, peroxidase, ascorbate, peroxisomal ascorbate peroxidase, PgAPX1, PHYPA_001206, PHYPA_001884, PHYPA_021776, PHYPA_024580, PHYPA_024582, Potri.002G081900, Potri.004G174500, Potri.005G112200, Potri.005G161900, Potri.005G179200, Potri.006G089000, Potri.006G132200, Potri.006G254500, Potri.009G015400, Potri.009G134100, Potri.016G084800, PpAPX, PpAPX-S, PpAPX2, PpAPX2.1, PpAPX2.2, PpAPX3, PpAPX6-related, PtAPX-S.1, PtAPX-S.2, PtAPX-TL29, PtAPX.3, PtAPX1.1, PtAPX1.2, PtAPX2, PtAPX3, PtAPX5, PtAPX5-like, PtAPX6 related, PtotAPX, RcAPX, sAPX, stromal APX, stromal ascorbate peroxidase, stromal ascorbate peroxidases, TaAPX, tAPX, thylakoid ascorbate peroxidase, thylakoid membrane-bound ascorbate peroxidases, thylakoid-bound ascorbate peroxidase

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.11 L-ascorbate peroxidase

General Stability

General Stability on EC 1.11.1.11 - L-ascorbate peroxidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis of crude enzyme preparations for 24 h against a buffer depleted of ascorbate or sorbitol results in 90% and 20% loss in enzyme activity, respectively. In air saturated solutions, salting-out results in a significant loss in enzyme activity, therefore exchange to N2 gas is performed prior to salting-out of the root enzyme
-
enzyme retains 35% activity after chymotrypsin digestion at pH 8 and 37°C for 40 min
loss of activity after incubation of crude extracts from pea shoots with either pronase or chymotrypsin
-
the enzyme is completely inactivated within 30 s in ascorbate-depleted medium under anaerobic conditions
-
the enzyme is relatively stable in ascorbate-depleted medium
Chlamydomonas sp.
the enzyme is very unstable in the absence of ascorbate and looses its activity within min particularly in the case of chloroplastic and mitochondrial isoforms
the half-inactivation time is estimated to be 15 min, when the enzyme is diluted with ascorbate-depleted medium
-
the recombinant enzyme-B remains active for at least 180 min after depletion of ascorbate
-
when the enzyme is diluted with the ascorbate-deleted medium, the half inactivation time is approximately 15 min
-