Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Citrus limon |
Crystallization (Comment) | Organism |
---|---|
construction of a 3-D structural model based on the crystal structure of Pisum sativum Apx, PDB code 1APX | Citrus limon |
General Stability | Organism |
---|---|
enzyme retains 35% activity after chymotrypsin digestion at pH 8 and 37°C for 40 min | Citrus limon |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
imidazole | enzyme shows a decrease in its activity with increasing imidazole concentration, approximately 50% activity is lost in the presence of 0.8 M imidazole | Citrus limon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
H2O2 | pH 7.4, 37°C | Citrus limon | |
0.4 | - |
L-ascorbate | pH 7.4, 37°C | Citrus limon |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28100 | - |
ESI Q-TOF | Citrus limon |
29000 | - |
1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein | Citrus limon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus limon | J7EIR8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | - |
Citrus limon | 2 monodehydroascorbate + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein | Citrus limon |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life 6.5 min | Citrus limon |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71.5 | - |
L-ascorbate | pH 7.4, 37°C | Citrus limon | |
84.5 | - |
H2O2 | pH 7.4, 37°C | Citrus limon |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Citrus limon |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Citrus limon | calculated | - |
5.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
178 | - |
L-ascorbate | pH 7.4, 37°C | Citrus limon | |
768 | - |
H2O2 | pH 7.4, 37°C | Citrus limon |