1.1.1.45: L-gulonate 3-dehydrogenase

This is an abbreviated version, for detailed information about L-gulonate 3-dehydrogenase, go to the full flat file.

Reaction

L-gulonate
+
NAD+
=
3-dehydro-L-gulonate
+
NADH
+
H+

Synonyms

GDH, L-3-aldonate dehydrogenase, L-3-aldonic dehydrogenase, L-3-hydroxyacid dehydrogenase, L-beta-hydroxy-acid-NAD-oxidoreductase, L-beta-hydroxyacid dehydrogenase, L-gulonate 3-dehydrogenase, L-gulonic acid dehydrogenase, More

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.45 L-gulonate 3-dehydrogenase

Engineering

Engineering on EC 1.1.1.45 - L-gulonate 3-dehydrogenase

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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D36R/Q41N
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
E157Q
-
mutation produces no large kinetic alterations
E97Q
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H145Q
-
inactive enzyme form
N195D
-
inactive enzyme form
N196Q
-
inactive enzyme form
Q41N
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
S124A
-
mutation decreases the catalytic efficiency 500 fold