Sequence of PAH1_YEAST
EC Number:3.1.3.4
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
phosphatidate phosphatase
P32567
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
862
95031
Reaction
a 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate
Other sequences found for EC No. 3.1.3.4
General information:
Sequence
0 MQYVGRALGS VSKTWSSINP ATLSGAIDVI VVEHPDGRLS CSPFHVRFGK FQILKPSQKK
60 VQVFINEKLS NMPMKLSDSG EAYFVFEMGD QVTDVPDELL VSPVMSATSS PPQSPETSIL
120 EGGTEGEGEG ENENKKKEKK VLEEPDFLDI NDTGDSGSKN SETTGSLSPT ESSTTTPPDS
180 VEERKLVEQR TKNFQQKLNK KLTEIHIPSK LDNNGDLLLD TEGYKPNKNM MHDTDIQLKQ
240 LLKDEFGNDS DISSFIKEDK NGNIKIVNPY EHLTDLSPPG TPPTMATSGS VLGLDAMESG
300 STLNSLSSSP SGSDTEDETS FSKEQSSKSE KTSKKGTAGS GETEKRYIRT IRLTNDQLKC
360 LNLTYGENDL KFSVDHGKAI VTSKLFVWRW DVPIVISDID GTITKSDALG HVLAMIGKDW
420 THLGVAKLFS EISRNGYNIL YLTARSAGQA DSTRSYLRSI EQNGSKLPNG PVILSPDRTM
480 AALRREVILK KPEVFKIACL NDIRSLYFED SDNEVDTEEK STPFFAGFGN RITDALSYRT
540 VGIPSSRIFT INTEGEVHME LLELAGYRSS YIHINELVDH FFPPVSLDSV DLRTNTSMVP
600 GSPPNRTLDN FDSEITSGRK TLFRGNQEEK FTDVNFWRDP LVDIDNLSDI SNDDSDNIDE
660 DTDVSQQSNI SRNRANSVKT AKVTKAPQRN VSGSTNNNEV LAASSDVENA SDLVSSHSSS
720 GSTPNKSTMS KGDIGKQIYL ELGSPLASPK LRYLDDMDDE DSNYNRTKSR RASSAAATSI
780 DKEFKKLSVS KAGAPTRIVS KINVSNDVHS LGNSDTESRR EQSVNETGRN QLPHNSMDDK
840 DLDSRVSDEF DDDEFDEDEF ED
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
201368
Irie K.,Takase M.,Araki H.,Oshima Y.
A gene, SMP2, involved in plasmid maintenance and respiration in Saccharomyces cerevisiae encodes a highly charged protein.
Mol. Gen. Genet.
236
283-288
1993
201369
Bowman S.,Churcher C.M.,Badcock K.,Brown D.,Chillingworth T.,Connor R.,Dedman K.,Devlin K.,Gentles S.,Hamlin N.,Hunt S.,Jagels K.,Lye G.,Moule S.,Odell C.,Pearson D.,Rajandream M.A.,Rice P.,Skelton J.,Walsh S.V.,Whitehead S.,Barrell B.G.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature
387
90-93
1997
201370
Engel S.R.,Dietrich F.S.,Fisk D.G.,Binkley G.,Balakrishnan R.,Costanzo M.C.,Dwight S.S.,Hitz B.C.,Karra K.,Nash R.S.,Weng S.,Wong E.D.,Lloyd P.,Skrzypek M.S.,Miyasato S.R.,Simison M.,Cherry J.M.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
G3 (Bethesda)
4
389-398
2014
201371
Huh W.-K.,Falvo J.V.,Gerke L.C.,Carroll A.S.,Howson R.W.,Weissman J.S.,O'Shea E.K.
Global analysis of protein localization in budding yeast.
Nature
425
686-691
2003
201372
Ghaemmaghami S.,Huh W.-K.,Bower K.,Howson R.W.,Belle A.,Dephoure N.,O'Shea E.K.,Weissman J.S.
Global analysis of protein expression in yeast.
Nature
425
737-741
2003
201373
Santos-Rosa H.,Leung J.,Grimsey N.,Peak-Chew S.,Siniossoglou S.
The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth.
EMBO J.
24
1931-1941
2005
201374
O'Hara L.,Han G.S.,Peak-Chew S.,Grimsey N.,Carman G.M.,Siniossoglou S.
Control of phospholipid synthesis by phosphorylation of the yeast lipin Pah1p/Smp2p Mg2+-dependent phosphatidate phosphatase.
J. Biol. Chem.
281
34537-34548
2006
201375
Han G.S.,Wu W.I.,Carman G.M.
The Saccharomyces cerevisiae Lipin homolog is a Mg2+-dependent phosphatidate phosphatase enzyme.
J. Biol. Chem.
281
9210-9218
2006
201376
Han G.S.,Siniossoglou S.,Carman G.M.
The cellular functions of the yeast lipin homolog PAH1p are dependent on its phosphatidate phosphatase activity.
J. Biol. Chem.
282
37026-37035
2007
201377
Li X.,Gerber S.A.,Rudner A.D.,Beausoleil S.A.,Haas W.,Villen J.,Elias J.E.,Gygi S.P.
Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.
J. Proteome Res.
6
1190-1197
2007
201378
Chi A.,Huttenhower C.,Geer L.Y.,Coon J.J.,Syka J.E.P.,Bai D.L.,Shabanowitz J.,Burke D.J.,Troyanskaya O.G.,Hunt D.F.
Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.
Proc. Natl. Acad. Sci. U.S.A.
104
2193-2198
2007
201379
Havriluk T.,Lozy F.,Siniossoglou S.,Carman G.M.
Colorimetric determination of pure Mg(2+)-dependent phosphatidate phosphatase activity.
Anal. Biochem.
373
392-394
2008
201380
Albuquerque C.P.,Smolka M.B.,Payne S.H.,Bafna V.,Eng J.,Zhou H.
A multidimensional chromatography technology for in-depth phosphoproteome analysis.
Mol. Cell. Proteomics
7
1389-1396
2008
201381
Holt L.J.,Tuch B.B.,Villen J.,Johnson A.D.,Gygi S.P.,Morgan D.O.
Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.
Science
325
1682-1686
2009
201382
Karanasios E.,Han G.S.,Xu Z.,Carman G.M.,Siniossoglou S.
A phosphorylation-regulated amphipathic helix controls the membrane translocation and function of the yeast phosphatidate phosphatase.
Proc. Natl. Acad. Sci. U.S.A.
107
17539-17544
2010
201383
Choi H.S.,Su W.M.,Morgan J.M.,Han G.S.,Xu Z.,Karanasios E.,Siniossoglou S.,Carman G.M.
Phosphorylation of phosphatidate phosphatase regulates its membrane association and physiological functions in Saccharomyces cerevisiae: identification of SER(602), THR(723), AND SER(744) as the sites phosphorylated by CDC28 (CDK1)-encoded cyclin-dependent kinase.
J. Biol. Chem.
286
1486-1498
2011
201384
Adeyo O.,Horn P.J.,Lee S.,Binns D.D.,Chandrahas A.,Chapman K.D.,Goodman J.M.
The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets.
J. Cell Biol.
192
1043-1055
2011
201385
Li T.Y.,Song L.,Sun Y.,Li J.,Yi C.,Lam S.M.,Xu D.,Zhou L.,Li X.,Yang Y.,Zhang C.S.,Xie C.,Huang X.,Shui G.,Lin S.Y.,Reue K.,Lin S.C.
Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.
Nat. Commun.
9
1916-1916
2018
