Sequence of PIF1_YEAST
EC Number:5.6.2.3
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
Couples ATP hydroysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
Other sequences found for EC No. 5.6.2.3
General information:
Sequence
0 MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF IQAQLKHPSI
60 LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED PVDKKPMRDK NVMNFINKDS
120 PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK KSRSTGFKNP LRPALKKESS FDELQNNSIS
180 QERSLEMINE NEKKKMQFGE KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE
240 QESIIKLAEN GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI
300 TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL LDKLDFIARK
360 IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK AWKEGVKMTI MLQKVFRQRG
420 DVKFIDMLNR MRLGNIDDET EREFKKLSRP LPDDEIIPAE LYSTRMEVER ANNSRLSKLP
480 GQVHIFNAID GGALEDEELK ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI
540 EFMDPETYFC YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE
600 GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS AGKRRLPLVR
660 FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW SLSIHKSQGQ TLPKVKVDLR
720 RVFEKGQAYV ALSRAVSREG LQVLNFDRTR IKAHQKVIDF YLTLSSAESA YKQLEADEQV
780 KKRKLDYAPG PKYKAKSKSK SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV
840 HSLVSDEPRG QDTEDHILE
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
205304
Foury F.,Lahaye A.
Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA.
EMBO J.
6
1441-1449
1987
205305
Bowman S.,Churcher C.M.,Badcock K.,Brown D.,Chillingworth T.,Connor R.,Dedman K.,Devlin K.,Gentles S.,Hamlin N.,Hunt S.,Jagels K.,Lye G.,Moule S.,Odell C.,Pearson D.,Rajandream M.A.,Rice P.,Skelton J.,Walsh S.V.,Whitehead S.,Barrell B.G.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature
387
90-93
1997
205306
Engel S.R.,Dietrich F.S.,Fisk D.G.,Binkley G.,Balakrishnan R.,Costanzo M.C.,Dwight S.S.,Hitz B.C.,Karra K.,Nash R.S.,Weng S.,Wong E.D.,Lloyd P.,Skrzypek M.S.,Miyasato S.R.,Simison M.,Cherry J.M.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
G3 (Bethesda)
4
389-398
2014
205307
Lahaye A.,Leterme S.,Foury F.
PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme.
J. Biol. Chem.
268
26155-26161
1993
205308
Lahaye A.,Stahl H.,Thines-Sempoux D.,Foury F.
PIF1: a DNA helicase in yeast mitochondria.
EMBO J.
10
997-1007
1991
205309
Schulz V.P.,Zakian V.A.
The Saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation.
Cell
76
145-155
1994
205310
Ivessa A.S.,Zhou J.-Q.,Zakian V.A.
The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA.
Cell
100
479-489
2000
205311
Zhou J.-Q.,Monson E.K.,Teng S.-C.,Schulz V.P.,Zakian V.A.
Pif1p helicase, a catalytic inhibitor of telomerase in yeast.
Science
289
771-774
2000
205312
Myung K.,Chen C.,Kolodner R.D.
Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae.
Nature
411
1073-1076
2001
205313
O'Rourke T.W.,Doudican N.A.,Mackereth M.D.,Doetsch P.W.,Shadel G.S.
Mitochondrial dysfunction due to oxidative mitochondrial DNA damage is reduced through cooperative actions of diverse proteins.
Mol. Cell. Biol.
22
4086-4093
2002
205314
Huh W.-K.,Falvo J.V.,Gerke L.C.,Carroll A.S.,Howson R.W.,Weissman J.S.,O'Shea E.K.
Global analysis of protein localization in budding yeast.
Nature
425
686-691
2003
205315
Ghaemmaghami S.,Huh W.-K.,Bower K.,Howson R.W.,Belle A.,Dephoure N.,O'Shea E.K.,Weissman J.S.
Global analysis of protein expression in yeast.
Nature
425
737-741
2003
205316
Sickmann A.,Reinders J.,Wagner Y.,Joppich C.,Zahedi R.P.,Meyer H.E.,Schoenfisch B.,Perschil I.,Chacinska A.,Guiard B.,Rehling P.,Pfanner N.,Meisinger C.
The proteome of Saccharomyces cerevisiae mitochondria.
Proc. Natl. Acad. Sci. U.S.A.
100
13207-13212
2003
205317
O'Rourke T.W.,Doudican N.A.,Zhang H.,Eaton J.S.,Doetsch P.W.,Shadel G.S.
Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability.
Gene
354
86-92
2005
205318
Doudican N.A.,Song B.,Shadel G.S.,Doetsch P.W.
Oxidative DNA damage causes mitochondrial genomic instability in Saccharomyces cerevisiae.
Mol. Cell. Biol.
25
5196-5204
2005
205319
Boule J.-B.,Vega L.R.,Zakian V.A.
The yeast Pif1p helicase removes telomerase from telomeric DNA.
Nature
438
57-61
2005
205320
Wagner M.,Price G.,Rothstein R.
The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae.
Genetics
174
555-573
2006
205321
Budd M.E.,Reis C.C.,Smith S.,Myung K.,Campbell J.L.
Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta.
Mol. Cell. Biol.
26
2490-2500
2006
205322
Eugster A.,Lanzuolo C.,Bonneton M.,Luciano P.,Pollice A.,Pulitzer J.F.,Stegberg E.,Berthiau A.-S.,Foerstemann K.,Corda Y.,Lingner J.,Geli V.,Gilson E.
The finger subdomain of yeast telomerase cooperates with Pif1p to limit telomere elongation.
Nat. Struct. Mol. Biol.
13
734-739
2006
205323
Cheng X.,Dunaway S.,Ivessa A.S.
The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial DNA.
Mitochondrion
7
211-222
2007
205324
Boule J.-B.,Zakian V.A.
The yeast Pif1p DNA helicase preferentially unwinds RNA-DNA substrates.
Nucleic Acids Res.
35
5809-5818
2007
205325
Vega L.R.,Phillips J.A.,Thornton B.R.,Benanti J.A.,Onigbanjo M.T.,Toczyski D.P.,Zakian V.A.
Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase.
PLoS Genet.
3
1065-1075
2007
205326
Albuquerque C.P.,Smolka M.B.,Payne S.H.,Bafna V.,Eng J.,Zhou H.
A multidimensional chromatography technology for in-depth phosphoproteome analysis.
Mol. Cell. Proteomics
7
1389-1396
2008
205327
Ribeyre C.,Lopes J.,Boule J.B.,Piazza A.,Guedin A.,Zakian V.A.,Mergny J.L.,Nicolas A.
The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo.
PLoS Genet.
5
0-0
2009
205328
Holt L.J.,Tuch B.B.,Villen J.,Johnson A.D.,Gygi S.P.,Morgan D.O.
Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.
Science
325
1682-1686
2009
205329
Barranco-Medina S.,Galletto R.
DNA binding induces dimerization of Saccharomyces cerevisiae Pif1.
Biochemistry
49
8445-8454
2010
205330
Cheng X.,Ivessa A.S.
Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial DNA.
Eur. J. Cell Biol.
89
742-747
2010
205331
Boule J.B.,Zakian V.A.
Characterization of the helicase activity and anti-telomerase properties of yeast Pif1p in vitro.
Methods Mol. Biol.
587
359-376
2010
205332
Paeschke K.,Capra J.A.,Zakian V.A.
DNA replication through G-quadruplex motifs is promoted by the Saccharomyces cerevisiae Pif1 DNA helicase.
Cell
145
678-691
2011
205333
Crider D.G.,Garcia-Rodriguez L.J.,Srivastava P.,Peraza-Reyes L.,Upadhyaya K.,Boldogh I.R.,Pon L.A.
Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression.
J. Cell Biol.
198
793-798
2012
205334
Ramanagoudr-Bhojappa R.,Chib S.,Byrd A.K.,Aarattuthodiyil S.,Pandey M.,Patel S.S.,Raney K.D.
Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA.
J. Biol. Chem.
288
16185-16195
2013
205335
Paeschke K.,Bochman M.L.,Garcia P.D.,Cejka P.,Friedman K.L.,Kowalczykowski S.C.,Zakian V.A.
Pif1 family helicases suppress genome instability at G-quadruplex motifs.
Nature
497
458-462
2013
205336
Ramanagoudr-Bhojappa R.,Blair L.P.,Tackett A.J.,Raney K.D.
Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein.
Nucleic Acids Res.
41
1029-1046
2013
205337
Galletto R.,Tomko E.J.
Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNA.
Nucleic Acids Res.
41
4613-4627
2013
