Sequence of LOVC_ASPTE
EC Number:2.3.1.161
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
9 malonyl-CoA + 11 NADPH + 10 H+ + S-adenosyl-L-methionine + holo-[lovastatin nonaketide synthase] = dihydromonacolin L-[lovastatin nonaketide synthase] + 9 CoA + 9 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
Other sequences found for EC No. 2.3.1.161
General information:
Sequence
0 MGDQPFIPPP QQTALTVNDH DEVTVWNAAP CPMLPRDQVY VRVEAVAINP SDTKMRGQFA
60 TPWAFLGTDY AGTVVAVGSD VTHIQVGDRV YGAQNEMCPR TPDQGAFSQY TVTRGRVWAK
120 IPKGLSFEQA AALPAGISTA GLAMKLLGLP LPSPSADQPP THSKPVYVLV YGGSTATATV
180 TMQMLRLSGY IPIATCSPHN FDLAKSRGAE EVFDYRAPNL AQTIRTYTKN NLRYALDCIT
240 NVESTTFCFA AIGRAGGHYV SLNPFPEHAA TRKMVTTDWT LGPTIFGEGS TWPAPYGRPG
300 SEEERQFGED LWRIAGQLVE DGRLVHHPLR VVQGGFDHIK QGMELVRKGE LSGEKLVVRL
360 EGP
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
820112
Kennedy J.,Auclair K.,Kendrew S.G.,Park C.,Vederas J.C.,Hutchinson C.R.
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
Science
284
1368-1372
1999
820113
Alberts A.W.,Chen J.,Kuron G.,Hunt V.,Huff J.,Hoffman C.,Rothrock J.,Lopez M.,Joshua H.,Harris E.,Patchett A.,Monaghan R.,Currie S.,Stapley E.,Albers-Schonberg G.,Hensens O.,Hirshfield J.,Hoogsteen K.,Liesch J.,Springer J.
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
Proc. Natl. Acad. Sci. U.S.A.
77
3957-3961
1980
820114
Hendrickson L.,Davis C.R.,Roach C.,Nguyen D.K.,Aldrich T.,McAda P.C.,Reeves C.D.
Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene.
Chem. Biol.
6
429-439
1999
820115
Sorensen J.L.,Auclair K.,Kennedy J.,Hutchinson C.R.,Vederas J.C.
Transformations of cyclic nonaketides by Aspergillus terreus mutants blocked for lovastatin biosynthesis at the lovA and lovC genes.
Org. Biomol. Chem.
1
50-59
2003
820116
Xie X.,Watanabe K.,Wojcicki W.A.,Wang C.C.,Tang Y.
Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.
Chem. Biol.
13
1161-1169
2006
820117
Xie X.,Pashkov I.,Gao X.,Guerrero J.L.,Yeates T.O.,Tang Y.
Rational improvement of simvastatin synthase solubility in Escherichia coli leads to higher whole-cell biocatalytic activity.
Biotechnol. Bioeng.
102
20-28
2009
820118
Gao X.,Xie X.,Pashkov I.,Sawaya M.R.,Laidman J.,Zhang W.,Cacho R.,Yeates T.O.,Tang Y.
Directed evolution and structural characterization of a simvastatin synthase.
Chem. Biol.
16
1064-1074
2009
820119
Xie X.,Meehan M.J.,Xu W.,Dorrestein P.C.,Tang Y.
Acyltransferase mediated polyketide release from a fungal megasynthase.
J. Am. Chem. Soc.
131
8388-8389
2009
820120
Ma S.M.,Li J.W.,Choi J.W.,Zhou H.,Lee K.K.,Moorthie V.A.,Xie X.,Kealey J.T.,Da Silva N.A.,Vederas J.C.,Tang Y.
Complete reconstitution of a highly reducing iterative polyketide synthase.
Science
326
589-592
2009
820121
Meehan M.J.,Xie X.,Zhao X.,Xu W.,Tang Y.,Dorrestein P.C.
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.
Biochemistry
50
287-299
2011
820122
Barriuso J.,Nguyen D.T.,Li J.W.,Roberts J.N.,MacNevin G.,Chaytor J.L.,Marcus S.L.,Vederas J.C.,Ro D.K.
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA.
J. Am. Chem. Soc.
133
8078-8081
2011
820123
Xu W.,Chooi Y.H.,Choi J.W.,Li S.,Vederas J.C.,Da Silva N.A.,Tang Y.
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
Angew. Chem. Int. Ed. Engl.
52
6472-6475
2013
820124
Jimenez-Oses G.,Osuna S.,Gao X.,Sawaya M.R.,Gilson L.,Collier S.J.,Huisman G.W.,Yeates T.O.,Tang Y.,Houk K.N.
The role of distant mutations and allosteric regulation on LovD active site dynamics.
Nat. Chem. Biol.
10
431-436
2014
820125
Chen M.C.,Tsai Y.C.,Tseng J.H.,Liou J.J.,Horng S.,Wen H.C.,Fan Y.C.,Zhong W.B.,Hsu S.P.
Simvastatin inhibits cell proliferation and migration in human anaplastic thyroid cancer.
Int. J. Mol. Sci.
18
0-0
2017
820126
Zhong W.B.,Tsai Y.C.,Chin L.H.,Tseng J.H.,Tang L.W.,Horng S.,Fan Y.C.,Hsu S.P.
A synergistic anti-cancer effect of troglitazone and lovastatin in a human anaplastic thyroid cancer cell line and in a mouse xenograft model.
Int. J. Mol. Sci.
19
0-0
2018
820127
Ames B.D.,Nguyen C.,Bruegger J.,Smith P.,Xu W.,Ma S.,Wong E.,Wong S.,Xie X.,Li J.W.,Vederas J.C.,Tang Y.,Tsai S.C.
Crystal structure and biochemical studies of the trans-acting polyketide enoyl reductase LovC from lovastatin biosynthesis.
Proc. Natl. Acad. Sci. U.S.A.
109
11144-11149
2012
