Sequence of LOVB_ASPTN
EC Number:2.3.1.161
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
lovastatin nonaketide synthase
Q0C8M3
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
3038
335023
Reaction
9 malonyl-CoA + 11 NADPH + 10 H+ + S-adenosyl-L-methionine + holo-[lovastatin nonaketide synthase] = dihydromonacolin L-[lovastatin nonaketide synthase] + 9 CoA + 9 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
Other sequences found for EC No. 2.3.1.161
General information:
Sequence
0 MAQSMYPNEP IVVVGSGCRF PGDANTPSKL WELLQHPRDV QSRIPKERFD VDTFYHPDGK
60 HHGRTNAPYA YVLQDDLGAF DAAFFNIQAG EAESMDPQHR LLLETVYEAV TNAGMRIQDL
120 QGTSTAVYVG VMTHDYETVS TRDLENIPTY SATGVAVSVA SNRISYFFDW HGPSMTIDTA
180 CSSSLVAVHL AVQQLRTGQS SMAIAAGANL ILGPMTFVLE SKLSMLSPSG RSRMWDAGAD
240 GYARGEAVCS VVLKTLSQAL RDGDTIECVI RETGVNQDGR TTGITMPNHS AQEALIKATY
300 AQAGLDITKA EDRCQFFEAH GTGTPAGDPQ EAEAIATAFF GHEQVARSDG NERAPLFVGS
360 AKTVVGHTEG TAGLAGLMKA SFAVRHGVIP PNLLFDKISP RVAPFYKNLR IPTEATQWPA
420 LPPGQPRRAS VNSFGFGGTN AHAIIEEYME PEQNQLRVSN NEDCPPMTGV LSLPLVLSAK
480 SQRSLKIMME EMLQFLETHP EIHLHDLTWS LLRKRSVLPF RRAIVGHSHE TIRRALEDAI
540 EDGIVSSDFT TEVRGQPSVL GIFTGQGAQW PGMLKNLIEA SPYVRSIVRE LDDSLQSLPE
600 KYRPSWTLLD QFMLEGEASN VQYASFSQPL CCAVQIVLVR LLEAARIRFT AVVGHSSGEI
660 ACAFAAGLIS ASVAIRIAYL RGVVSAGGAR GTPGAMLAAG MSFEEAQEIC ELDAFEGRIC
720 VAASNSPDSV TFSGDANAID HLKSMLEDES TFARLLRVDT AYHSHHMLPC ADPYMQALEE
780 CGCAVADAGS PAGSVPWYSS VNAENRQMAA RDVTAEYWKD NLVSPVLFSH AVQRAVVTHK
840 ALDIGIEVGC HPALKSPCVA TIKDVLSGVD LAYTGCLERG KNDLDTFSRA LAYLWERFGA
900 SSFDADEFMR AVAPDRPCMS VSKLLPAYPW DHSRRYWVES RATRHHLRGP KPHLLLGKLS
960 EYSTPLSFQW LNFVRPRDIE WLDGHALQGQ TVFPAAGYIV MAMEAALMIA GTHAKQVQLL
1020 EILDMSIDKA VIFDDEDSLV ELNLTADVSR NAGEAGSMTI SFKIDSCLSK EGNLSLSAKG
1080 QLALMIGDVN SRTTSASDQH HLPPPEEEHP HMNRVNIKAF YHELGLMGYN YSKDFRRLHN
1140 MQRADLRASG TIDFIPLMDE GNGCPLLLHP ASLDVAFQTV IGAYSSPGDR RLRCLYVPTH
1200 VDRITLVPSL CLATAESGCE KVAFNTINTY DKGDYLSGDI AVFDAEQTTL FQVENITFKP
1260 FSPPDASTDH AMFARWSWGP LTPDSLLDNP EYWATAQDKE AIPIIERIVY FYIRSFLNQL
1320 TLEERQKAAF HLQKQIEWLE QVLASAKEGR HLWYDPGWEN DTEAQIEHLC TANSYHPHVR
1380 LVQRVGQHLL PTVRSNGNPF DLLDHDGLLT EFYTNTLSFG PALHYARELV AQIAHRYQSM
1440 DILEIGAGTG GATKYVLATP QLGFNSYTYT DISTGFFEQA REQFAPFEDR MVFEPLDIRR
1500 SPAEQGFETH AYDLIIASNV LHATPDLEKT MAHARSLLKP GGQMVILEIT HKEHTRLGFI
1560 FGLFADWWAG VDDGRCTEPF VSFDRWDAIL KRVGFSGVDS RTTDRDANLF PTSVFSTHAI
1620 DATVEYLDAP LASSGTVKDS YPPLVVVGGQ TPKSQRLLND IKAIMPPRPL QTYKRLVDLL
1680 DAEELPMKST FVMLTELDEE LFAGLTEETF EATKLLLTYS SNTVWLTENA WVQHPHQAST
1740 IGMLRSIRRE HPDLGVHVLD VDAVETFDAT FLVEQVLRLE EHTDELASST TWTQEPEVSW
1800 CKGRPWIPRL KRDLARNNRM NSSRRPIYEM IDSSRAPVAL QTAPDSSSYF LESAETWFVP
1860 ESVRQMETKT VYVHFSCPHA LRVGQLGFFY LVQGHVQEGN REVPVVALAE RNASIVHVRP
1920 DYIYTEADNN LSEGGGSLIV TVLAAAVLAE TVISTAKSLG VTDSILVLNP PSICGQMLLH
1980 AGEEIGLQVH LATTSGNRSS VSAGDAKSWL TLHARDTDWH LRRVLPRGVQ AFVDLSADQS
2040 CECLTQRMMK VLMPGCAHYR AADLFTDTVS TELHRGLRHQ ASLPAAYWEH VVSLARQGLS
2100 SVSEGWEVMP CTQFAAHADK TRPDLSTVIS WPRESDKATL PTRVRSIDAE TLFAADKTYL
2160 LVGLTGDLGR SLGRWMVQHG ACHIVLTSRN PQVNPKWLAH VEELGGRVTV LSMDVTSENS
2220 VDAGLAKIKD LHLPPVGGIA FGPLVLQDVM LKNMELPMME MVLNPKVEGV RILHEKFSDP
2280 TSSNPLDFFV MFSSIVAVMG NPGQANYSAA NCYLQALAQQ RVASGLAAST IDIGAVYGVG
2340 FVTRAELEED FNAIRFMFDS VEEHELHTLF AEAVVAGRRA VHQQEQQRKF ATVLDMADLE
2400 LTTGIPPLDP ALKDRITFFD DPRIGNLKIP EYRGAKAGEG AAGSKGSVKE QLLQATNLDQ
2460 VRQIVIDGLS AKLQVTLQIP DGESVHPTIS LIDQGVDSLG AVTVGTWFSK QLYLDLPLLK
2520 VLGGASIADL ADEAAARLPP SSIPLVAATD GGAESTDNTS ENEVSGREDT DLSAAATITE
2580 PSSADEDDTE PGDEDVPRSH HPLSLGQEYS WRIQQGAEDP TVFNNTIGMF MKGPIDLKRL
2640 YKALRAVLRR HEIFRTGFAN VDENGMAQLV FGQTKNKVQT IQVSDRAGAE EGYRQLVQTR
2700 YNPAAGDTLR LVDFFWGQDD HLLVVAYHRL VGDGSTTENI FVEAGQLYDG RSLSPRVPQF
2760 ADLAARQRAM LEDGRMEEDL AYWKEMHQRP SSIPVLPLMR PLVGNSSTSN TPNFQHCGSW
2820 QQHEAVARLD PMVAFRIKER SRKHKATPMQ FYLAAYQVLL ARLTDSTDLT VGLADTNRAT
2880 VDEMAAMGFF ANLLPLRFRD FRPHITFGEH LIATRDLVRE ALQHARVPYG VLLDQLGLEV
2940 PVPTSNQPAP LLQAVFDYKQ GQAESGTIGG AKITEVIATR ERTPYDVVLE MSDDPTKDPL
3000 LTAKLQSSRY EAHHPQAFLE SYMSLLSMFS MNPALKLA
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
820095
Hendrickson L.,Davis C.R.,Roach C.,Nguyen D.K.,Aldrich T.,McAda P.C.,Reeves C.D.
Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene.
Chem. Biol.
6
429-439
1999
820096
Alberts A.W.,Chen J.,Kuron G.,Hunt V.,Huff J.,Hoffman C.,Rothrock J.,Lopez M.,Joshua H.,Harris E.,Patchett A.,Monaghan R.,Currie S.,Stapley E.,Albers-Schonberg G.,Hensens O.,Hirshfield J.,Hoogsteen K.,Liesch J.,Springer J.
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
Proc. Natl. Acad. Sci. U.S.A.
77
3957-3961
1980
820097
Kennedy J.,Auclair K.,Kendrew S.G.,Park C.,Vederas J.C.,Hutchinson C.R.
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
Science
284
1368-1372
1999
820098
Sorensen J.L.,Auclair K.,Kennedy J.,Hutchinson C.R.,Vederas J.C.
Transformations of cyclic nonaketides by Aspergillus terreus mutants blocked for lovastatin biosynthesis at the lovA and lovC genes.
Org. Biomol. Chem.
1
50-59
2003
820099
Xie X.,Watanabe K.,Wojcicki W.A.,Wang C.C.,Tang Y.
Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.
Chem. Biol.
13
1161-1169
2006
820100
Xie X.,Pashkov I.,Gao X.,Guerrero J.L.,Yeates T.O.,Tang Y.
Rational improvement of simvastatin synthase solubility in Escherichia coli leads to higher whole-cell biocatalytic activity.
Biotechnol. Bioeng.
102
20-28
2009
820101
Gao X.,Xie X.,Pashkov I.,Sawaya M.R.,Laidman J.,Zhang W.,Cacho R.,Yeates T.O.,Tang Y.
Directed evolution and structural characterization of a simvastatin synthase.
Chem. Biol.
16
1064-1074
2009
820102
Xie X.,Meehan M.J.,Xu W.,Dorrestein P.C.,Tang Y.
Acyltransferase mediated polyketide release from a fungal megasynthase.
J. Am. Chem. Soc.
131
8388-8389
2009
820103
Ma S.M.,Li J.W.,Choi J.W.,Zhou H.,Lee K.K.,Moorthie V.A.,Xie X.,Kealey J.T.,Da Silva N.A.,Vederas J.C.,Tang Y.
Complete reconstitution of a highly reducing iterative polyketide synthase.
Science
326
589-592
2009
820104
Meehan M.J.,Xie X.,Zhao X.,Xu W.,Tang Y.,Dorrestein P.C.
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.
Biochemistry
50
287-299
2011
820105
Barriuso J.,Nguyen D.T.,Li J.W.,Roberts J.N.,MacNevin G.,Chaytor J.L.,Marcus S.L.,Vederas J.C.,Ro D.K.
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA.
J. Am. Chem. Soc.
133
8078-8081
2011
820106
Ames B.D.,Nguyen C.,Bruegger J.,Smith P.,Xu W.,Ma S.,Wong E.,Wong S.,Xie X.,Li J.W.,Vederas J.C.,Tang Y.,Tsai S.C.
Crystal structure and biochemical studies of the trans-acting polyketide enoyl reductase LovC from lovastatin biosynthesis.
Proc. Natl. Acad. Sci. U.S.A.
109
11144-11149
2012
820107
Boettger D.,Bergmann H.,Kuehn B.,Shelest E.,Hertweck C.
Evolutionary imprint of catalytic domains in fungal PKS-NRPS hybrids.
ChemBioChem
13
2363-2373
2012
820108
Xu W.,Chooi Y.H.,Choi J.W.,Li S.,Vederas J.C.,Da Silva N.A.,Tang Y.
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
Angew. Chem. Int. Ed. Engl.
52
6472-6475
2013
820109
Jimenez-Oses G.,Osuna S.,Gao X.,Sawaya M.R.,Gilson L.,Collier S.J.,Huisman G.W.,Yeates T.O.,Tang Y.,Houk K.N.
The role of distant mutations and allosteric regulation on LovD active site dynamics.
Nat. Chem. Biol.
10
431-436
2014
820110
Chen M.C.,Tsai Y.C.,Tseng J.H.,Liou J.J.,Horng S.,Wen H.C.,Fan Y.C.,Zhong W.B.,Hsu S.P.
Simvastatin inhibits cell proliferation and migration in human anaplastic thyroid cancer.
Int. J. Mol. Sci.
18
0-0
2017
820111
Zhong W.B.,Tsai Y.C.,Chin L.H.,Tseng J.H.,Tang L.W.,Horng S.,Fan Y.C.,Hsu S.P.
A synergistic anti-cancer effect of troglitazone and lovastatin in a human anaplastic thyroid cancer cell line and in a mouse xenograft model.
Int. J. Mol. Sci.
19
0-0
2018
