Sequence of LOVB_ASPTE
EC Number:2.3.1.161
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
9 malonyl-CoA + 11 NADPH + 10 H+ + S-adenosyl-L-methionine + holo-[lovastatin nonaketide synthase] = dihydromonacolin L-[lovastatin nonaketide synthase] + 9 CoA + 9 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
Other sequences found for EC No. 2.3.1.161
General information:
Sequence
0 MAQSMYPNEP IVVVGSGCRF PGDANTPSKL WELLQHPRDV QSRIPKERFD VDTFYHPDGK
60 HHGRTNAPYA YVLQDDLGAF DAAFFNIQAG EAESMDPQHR LLLETVYEAV TNAGMRIQDL
120 QGTSTAVYVG VMTHDYETVS TRDLESIPTY SATGVAVSVA SNRISYFFDW HGPSMTIDTA
180 CSSSLVAVHL AVQQLRTGQS SMAIAAGANL ILGPMTFVLE SKLSMLSPSG RSRMWDAGAD
240 GYARGEAVCS VVLKTLSQAL RDGDTIECVI RETGVNQDGR TTGITMPNHS AQEALIKATY
300 AQAGLDITKA EDRCQFFEAH GTGTPAGDPQ EAEAIATAFF GHEQVARSDG NERAPLFVGS
360 AKTVVGHTEG TAGLAGLMKA SFAVRHGVIP PNLLFDKISP RVAPFYKNLR IPTEATQWPA
420 LPPGQPRRAS VNSFGFGGTN AHAIIEEYME PEQNQLRVSN NEDCPPMTGV LSLPLVLSAK
480 SQRSLKIMME EMLQFLQSHP EIHLHDLTWS LLRKRSVLPF RRAIVGHSHE TIRRALEDAI
540 EDGIVSSDFT TEVRGQPSVL GIFTGQGAQW PGMLKNLIEA SPYVRNIVRE LDDSLQSLPE
600 KYRPSWTLLD QFMLEGEASN VQYATFSQPL CCAVQIVLVR LLEAARIRFT AVVGHSSGEI
660 ACAFAAGLIS ASLAIRIAYL RGVVSAGGAR GTPGAMLAAG MSFEEAQEIC ELDAFEGRIC
720 VAASNSPDSV TFSGDANAID HLKGMLEDES TFARLLKVDT AYHSHHMLPC ADPYMQALEE
780 CGCAVADAGS PAGSVPWYSS VDAENRQMAA RDVTAKYWKD NLVSPVLFSH AVQRAVVTHK
840 ALDIGIEVGC HPALKSPCVA TIKDVLSGVD LAYTGCLERG KNDLDSFSRA LAYLWERFGA
900 SSFDADEFMR AVAPDRPCMS VSKLLPAYPW DRSRRYWVES RATRHHLRGP KPHLLLGKLS
960 EYSTPLSFQW LNFVRPRDIE WLDGHALQGQ TVFPAAGYIV MAMEAALMIA GTHAKQVKLL
1020 EILDMSIDKA VIFDDEDSLV ELNLTADVSR NAGEAGSMTI SFKIDSCLSK EGNLSLSAKG
1080 QLALTIEDVN PRTTSASDQH HLPPPEEEHP HMNRVNINAF YHELGLMGYN YSKDFRRLHN
1140 MQRADLRASG TLDFIPLMDE GNGCPLLLHP ASLDVAFQTV IGAYSSPGDR RLRCLYVPTH
1200 VDRITLVPSL CLATAESGCE KVAFNTINTY DKGDYLSGDI VVFDAEQTTL FQVENITFKP
1260 FSPPDASTDH AMFARWSWGP LTPDSLLDNP EYWATAQDKE AIPIIERIVY FYIRSFLSQL
1320 TLEERQQAAF HLQKQIEWLE QVLASAKEGR HLWYDPGWEN DTEAQIEHLC TANSYHPHVR
1380 LVQRVGQHLL PTVRSNGNPF DLLDHDGLLT EFYTNTLSFG PALHYARELV AQIAHRYQSM
1440 DILEIGAGTG GATKYVLATP QLGFNSYTYT DISTGFFEQA REQFAPFEDR MVFEPLDIRR
1500 SPAEQGFEPH AYDLIIASNV LHATPDLEKT MAHARSLLKP GGQMVILEIT HKEHTRLGFI
1560 FGLFADWWAG VDDGRCTEPF VSFDRWDAIL KRVGFSGVDS RTTDRDANLF PTSVFSTHAI
1620 DATVEYLDAP LASSGTVKDS YPPLVVVGGQ TPQSQRLLND IKAIMPPRPL QTYKRLVDLL
1680 DAEELPMKST FVMLTELDEE LFAGLTEETF EATKLLLTYA SNTVWLTENA WVQHPHQAST
1740 IGMLRSIRRE HPDLGVHVLD VDAVETFDAT FLVEQVLRLE EHTDELASST TWTQEPEVSW
1800 CKGRPWIPRL KRDLARNNRM NSSRRPIYEM IDSSRAPVAL QTARDSSSYF LESAETWFVP
1860 ESVQQMETKT IYVHFSCPHA LRVGQLGFFY LVQGHVQEGN REVPVVALAE RNASIVHVRP
1920 DYIYTEADNN LSEGGGSLMV TVLAAAVLAE TVISTAKCLG VTDSILVLNP PSICGQMLLH
1980 AGEEIGLQVH LATTSGNRSS VSAGDAKSWL TLHARDTDWH LRRVLPRGVQ ALVDLSADQS
2040 CEGLTQRMMK VLMPGCAHYR AADLFTDTVS TELHSGSRHQ ASLPAAYWEH VVSLARQGLP
2100 SVSEGWEVMP CTQFAAHADK TRPDLSTVIS WPRESDEATL PTRVRSIDAE TLFAADKTYL
2160 LVGLTGDLGR SLGRWMVQHG ACHIVLTSRN PQVNPKWLAH VEELGGRVTV LSMDVTSQNS
2220 VEAGLAKLKD LHLPPVGGIA FGPLVLQDVM LNNMELPMME MVLNPKVEGV RILHEKFSDP
2280 TSSNPLDFFV MFSSIVAVMG NPGQANYSAA NCYLQALAQQ RVASGLAAST IDIGAVYGVG
2340 FVTRAELEED FNAIRFMFDS VEEHELHTLF AEAVVAGRRA VHQQEQQRKF ATVLDMADLE
2400 LTTGIPPLDP ALKDRITFFD DPRIGNLKIP EYRGAKAGEG AAGSKGSVKE QLLQATNLDQ
2460 VRQIVIDGLS AKLQVTLQIP DGESVHPTIP LIDQGVDSLG AVTVGTWFSK QLYLDLPLLK
2520 VLGGASITDL ANEAAARLPP SSIPLVAATD GGAESTDNTS ENEVSGREDT DLSAAATITE
2580 PSSADEDDTE PGDEDVPRSH HPLSLGQEYS WRIQQGAEDP TVFNNTIGMF MKGSIDLKRL
2640 YKALRAVLRR HEIFRTGFAN VDENGMAQLV FGQTKNKVQT IQVSDRAGAE EGYRQLVQTR
2700 YNPAAGDTLR LVDFFWGQDD HLLVVAYHRL VGDGSTTENI FVEAGQLYDG TSLSPHVPQF
2760 ADLAARQRAM LEDGRMEEDL AYWKKMHYRP SSIPVLPLMR PLVGNSSRSD TPNFQHCGPW
2820 QQHEAVARLD PMVAFRIKER SRKHKATPMQ FYLAAYQVLL ARLTDSTDLT VGLADTNRAT
2880 VDEMAAMGFF ANLLPLRFRD FRPHITFGEH LIATRDLVRE ALQHARVPYG VLLDQLGLEV
2940 PVPTSNQPAP LFQAVFDYKQ GQAESGTIGG AKITEVIATR ERTPYDVVLE MSDDPTKDPL
3000 LTAKLQSSRY EAHHPQAFLE SYMSLLSMFS MNPALKLA
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
820077
Hendrickson L.,Davis C.R.,Roach C.,Nguyen D.K.,Aldrich T.,McAda P.C.,Reeves C.D.
Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene.
Chem. Biol.
6
429-439
1999
820078
Alberts A.W.,Chen J.,Kuron G.,Hunt V.,Huff J.,Hoffman C.,Rothrock J.,Lopez M.,Joshua H.,Harris E.,Patchett A.,Monaghan R.,Currie S.,Stapley E.,Albers-Schonberg G.,Hensens O.,Hirshfield J.,Hoogsteen K.,Liesch J.,Springer J.
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
Proc. Natl. Acad. Sci. U.S.A.
77
3957-3961
1980
820079
Kennedy J.,Auclair K.,Kendrew S.G.,Park C.,Vederas J.C.,Hutchinson C.R.
Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.
Science
284
1368-1372
1999
820080
Sorensen J.L.,Auclair K.,Kennedy J.,Hutchinson C.R.,Vederas J.C.
Transformations of cyclic nonaketides by Aspergillus terreus mutants blocked for lovastatin biosynthesis at the lovA and lovC genes.
Org. Biomol. Chem.
1
50-59
2003
820081
Xie X.,Watanabe K.,Wojcicki W.A.,Wang C.C.,Tang Y.
Biosynthesis of lovastatin analogs with a broadly specific acyltransferase.
Chem. Biol.
13
1161-1169
2006
820082
Xie X.,Pashkov I.,Gao X.,Guerrero J.L.,Yeates T.O.,Tang Y.
Rational improvement of simvastatin synthase solubility in Escherichia coli leads to higher whole-cell biocatalytic activity.
Biotechnol. Bioeng.
102
20-28
2009
820083
Gao X.,Xie X.,Pashkov I.,Sawaya M.R.,Laidman J.,Zhang W.,Cacho R.,Yeates T.O.,Tang Y.
Directed evolution and structural characterization of a simvastatin synthase.
Chem. Biol.
16
1064-1074
2009
820084
Xie X.,Meehan M.J.,Xu W.,Dorrestein P.C.,Tang Y.
Acyltransferase mediated polyketide release from a fungal megasynthase.
J. Am. Chem. Soc.
131
8388-8389
2009
820085
Ma S.M.,Li J.W.,Choi J.W.,Zhou H.,Lee K.K.,Moorthie V.A.,Xie X.,Kealey J.T.,Da Silva N.A.,Vederas J.C.,Tang Y.
Complete reconstitution of a highly reducing iterative polyketide synthase.
Science
326
589-592
2009
820086
Meehan M.J.,Xie X.,Zhao X.,Xu W.,Tang Y.,Dorrestein P.C.
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.
Biochemistry
50
287-299
2011
820087
Barriuso J.,Nguyen D.T.,Li J.W.,Roberts J.N.,MacNevin G.,Chaytor J.L.,Marcus S.L.,Vederas J.C.,Ro D.K.
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA.
J. Am. Chem. Soc.
133
8078-8081
2011
820088
Boettger D.,Bergmann H.,Kuehn B.,Shelest E.,Hertweck C.
Evolutionary imprint of catalytic domains in fungal PKS-NRPS hybrids.
ChemBioChem
13
2363-2373
2012
820089
Ames B.D.,Nguyen C.,Bruegger J.,Smith P.,Xu W.,Ma S.,Wong E.,Wong S.,Xie X.,Li J.W.,Vederas J.C.,Tang Y.,Tsai S.C.
Crystal structure and biochemical studies of the trans-acting polyketide enoyl reductase LovC from lovastatin biosynthesis.
Proc. Natl. Acad. Sci. U.S.A.
109
11144-11149
2012
820090
Xu W.,Chooi Y.H.,Choi J.W.,Li S.,Vederas J.C.,Da Silva N.A.,Tang Y.
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
Angew. Chem. Int. Ed. Engl.
52
6472-6475
2013
820091
Jimenez-Oses G.,Osuna S.,Gao X.,Sawaya M.R.,Gilson L.,Collier S.J.,Huisman G.W.,Yeates T.O.,Tang Y.,Houk K.N.
The role of distant mutations and allosteric regulation on LovD active site dynamics.
Nat. Chem. Biol.
10
431-436
2014
820092
Chen M.C.,Tsai Y.C.,Tseng J.H.,Liou J.J.,Horng S.,Wen H.C.,Fan Y.C.,Zhong W.B.,Hsu S.P.
Simvastatin inhibits cell proliferation and migration in human anaplastic thyroid cancer.
Int. J. Mol. Sci.
18
0-0
2017
820093
Zhong W.B.,Tsai Y.C.,Chin L.H.,Tseng J.H.,Tang L.W.,Horng S.,Fan Y.C.,Hsu S.P.
A synergistic anti-cancer effect of troglitazone and lovastatin in a human anaplastic thyroid cancer cell line and in a mouse xenograft model.
Int. J. Mol. Sci.
19
0-0
2018
