Sequence of IPNA_PENRW
EC Number:1.21.3.1
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
isopenicillin-N synthase
B6HLU0
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)
331
38010
Reaction
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O
Other sequences found for EC No. 1.21.3.1
General information:
Sequence
0 MASTPKANVP KIDVSPLFGD NMEEKMKVAR AIDAASRDTG FFYAVNHGVD VKRLSNKTRE
60 FHFSITDEEK WDLAIRAYNK EHQDQIRAGY YLSIPEKKAV ESFCYLNPNF KPDHPLIQSK
120 TPTHEVNVWP DEKKHPGFRE FAEQYYWDVF GLSSALLRGY ALALGKEEDF FSRHFKKEDA
180 LSSVVLIRYP YLNPYPPAAI KTAEDGTKLS FEWHEDVSLI TVLYQSDVAN LQVEMPQGYL
240 DIEADDNAYL VNCGSYMAHI TNNYYPAPIH RVKWVNEERQ SLPFFVNLGF NDTVQPWDPS
300 KEDGKTDQRP ISYGDYLQNG LVSLINKNGQ T
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
686544
van den Berg M.A.,Albang R.,Albermann K.,Badger J.H.,Daran J.-M.,Driessen A.J.M.,Garcia-Estrada C.,Fedorova N.D.,Harris D.M.,Heijne W.H.M.,Joardar V.S.,Kiel J.A.K.W.,Kovalchuk A.,Martin J.F.,Nierman W.C.,Nijland J.G.,Pronk J.T.,Roubos J.A.,van der Klei I.J.,van Peij N.N.M.E.,Veenhuis M.,von Doehren H.,Wagner C.,Wortman J.R.,Bovenberg R.A.L.
Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum.
Nat. Biotechnol.
26
1161-1168
2008
686545
Revilla G.,Ramos F.R.,Lopez-Nieto M.J.,Alvarez E.,Martin J.F.
Glucose represses formation of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine and isopenicillin N synthase but not penicillin acyltransferase in Penicillium chrysogenum.
J. Bacteriol.
168
947-952
1986
686546
Smith D.J.,Burnham M.K.,Edwards J.,Earl A.J.,Turner G.
Cloning and heterologous expression of the penicillin biosynthetic gene cluster from penicillum chrysogenum.
Biotechnology (N.Y.)
8
39-41
1990
686547
Whiteman P.A.,Abraham E.P.,Baldwin J.E.,Fleming M.D.,Schofield C.J.,Sutherland J.D.,Willis A.C.
Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans.
FEBS Lett.
262
342-344
1990
686548
Tobin M.B.,Fleming M.D.,Skatrud P.L.,Miller J.R.
Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli.
J. Bacteriol.
172
5908-5914
1990
686549
Mueller W.H.,van der Krift T.P.,Krouwer A.J.,Woesten H.A.,van der Voort L.H.,Smaal E.B.,Verkleij A.J.
Localization of the pathway of the penicillin biosynthesis in Penicillium chrysogenum.
EMBO J.
10
489-495
1991
686550
Bainbridge Z.A.,Scott R.I.,Perry D.
Oxygen utilisation by isopenicillin N synthase from Penicillium chrysogenum.
J. Chem. Technol. Biotechnol.
55
233-238
1992
686551
Huffman G.W.,Gesellchen P.D.,Turner J.R.,Rothenberger R.B.,Osborne H.E.,Miller F.D.,Chapman J.L.,Queener S.W.
Substrate specificity of isopenicillin N synthase.
J. Med. Chem.
35
1897-1914
1992
686552
Lendenfeld T.,Ghali D.,Wolschek M.,Kubicek-Pranz E.M.,Kubicek C.P.
Subcellular compartmentation of penicillin biosynthesis in Penicillium chrysogenum. The amino acid precursors are derived from the vacuole.
J. Biol. Chem.
268
665-671
1993
686553
Suarez T.,Penalva M.A.
Characterization of a Penicillium chrysogenum gene encoding a PacC transcription factor and its binding sites in the divergent pcbAB-pcbC promoter of the penicillin biosynthetic cluster.
Mol. Microbiol.
20
529-540
1996
686554
Etchegaray A.,Dieckmann R.,Kennedy J.,Turner G.,von Doehren H.
ACV synthetase: expression of amino acid activating domains of the Penicillium chrysogenum enzyme in Aspergillus nidulans.
Biochem. Biophys. Res. Commun.
237
166-169
1997
686555
Theilgaard H.B.,Kristiansen K.N.,Henriksen C.M.,Nielsen J.
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
Biochem. J.
327
185-191
1997
686556
van der Lende T.R.,van de Kamp M.,Berg M.,Sjollema K.,Bovenberg R.A.,Veenhuis M.,Konings W.N.,Driessen A.J.
delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine synthetase, that mediates the first committed step in penicillin biosynthesis, is a cytosolic enzyme.
Fungal Genet. Biol.
37
49-55
2002
686557
Siewers V.,Chen X.,Huang L.,Zhang J.,Nielsen J.
Heterologous production of non-ribosomal peptide LLD-ACV in Saccharomyces cerevisiae.
Metab. Eng.
11
391-397
2009
686558
Wu X.,Garcia-Estrada C.,Vaca I.,Martin J.F.
Motifs in the C-terminal region of the Penicillium chrysogenum ACV synthetase are essential for valine epimerization and processivity of tripeptide formation.
Biochimie
94
354-364
2012
686559
Dominguez-Santos R.,Martin J.F.,Kosalkova K.,Prieto C.,Ullan R.V.,Garcia-Estrada C.
The regulatory factor PcRFX1 controls the expression of the three genes of beta-lactam biosynthesis in Penicillium chrysogenum.
Fungal Genet. Biol.
49
866-881
2012
686560
Fernandez-Aguado M.,Teijeira F.,Martin J.F.,Ullan R.V.
A vacuolar membrane protein affects drastically the biosynthesis of the ACV tripeptide and the beta-lactam pathway of Penicillium chrysogenum.
Appl. Microbiol. Biotechnol.
97
795-808
2013
686561
Fernandez-Aguado M.,Ullan R.V.,Teijeira F.,Rodriguez-Castro R.,Martin J.F.
The transport of phenylacetic acid across the peroxisomal membrane is mediated by the PaaT protein in Penicillium chrysogenum.
Appl. Microbiol. Biotechnol.
97
3073-3084
2013
686562
Fernandez-Aguado M.,Martin J.F.,Rodriguez-Castro R.,Garcia-Estrada C.,Albillos S.M.,Teijeira F.,Ullan R.V.
New insights into the isopenicillin N transport in Penicillium chrysogenum.
Metab. Eng.
22
89-103
2014
