Sequence of IPNA_EMENI

isopenicillin-N synthase

P05326

331

37522

686504

Ramon D.,Carramolino L.,Patino C.,Sanchez F.,Penalva M.A.

Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the beta-lactam ring in Aspergillus nidulans.

Gene

57

171-181

1987

686505

Weigel B.J.,Burgett S.G.,Chen V.J.,Skatrud P.L.,Frolik C.A.,Queener S.W.,Ingolia T.D.

Cloning and expression in Escherichia coli of isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans.

J. Bacteriol.

170

3817-3826

1988

686506

Galagan J.E.,Calvo S.E.,Cuomo C.,Ma L.-J.,Wortman J.R.,Batzoglou S.,Lee S.-I.,Bastuerkmen M.,Spevak C.C.,Clutterbuck J.,Kapitonov V.,Jurka J.,Scazzocchio C.,Farman M.L.,Butler J.,Purcell S.,Harris S.,Braus G.H.,Draht O.,Busch S.,D'Enfert C.,Bouchier C.,Goldman G.H.,Bell-Pedersen D.,Griffiths-Jones S.,Doonan J.H.,Yu J.,Vienken K.,Pain A.,Freitag M.,Selker E.U.,Archer D.B.,Penalva M.A.,Oakley B.R.,Momany M.,Tanaka T.,Kumagai T.,Asai K.,Machida M.,Nierman W.C.,Denning D.W.,Caddick M.X.,Hynes M.,Paoletti M.,Fischer R.,Miller B.L.,Dyer P.S.,Sachs M.S.,Osmani S.A.,Birren B.W.

Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae.

Nature

438

1105-1115

2005

686507

Wortman J.R.,Gilsenan J.M.,Joardar V.,Deegan J.,Clutterbuck J.,Andersen M.R.,Archer D.,Bencina M.,Braus G.,Coutinho P.,von Dohren H.,Doonan J.,Driessen A.J.,Durek P.,Espeso E.,Fekete E.,Flipphi M.,Estrada C.G.,Geysens S.,Goldman G.,de Groot P.W.,Hansen K.,Harris S.D.,Heinekamp T.,Helmstaedt K.,Henrissat B.,Hofmann G.,Homan T.,Horio T.,Horiuchi H.,James S.,Jones M.,Karaffa L.,Karanyi Z.,Kato M.,Keller N.,Kelly D.E.,Kiel J.A.,Kim J.M.,van der Klei I.J.,Klis F.M.,Kovalchuk A.,Krasevec N.,Kubicek C.P.,Liu B.,Maccabe A.,Meyer V.,Mirabito P.,Miskei M.,Mos M.,Mullins J.,Nelson D.R.,Nielsen J.,Oakley B.R.,Osmani S.A.,Pakula T.,Paszewski A.,Paulsen I.,Pilsyk S.,Pocsi I.,Punt P.J.,Ram A.F.,Ren Q.,Robellet X.,Robson G.,Seiboth B.,van Solingen P.,Specht T.,Sun J.,Taheri-Talesh N.,Takeshita N.,Ussery D.,vanKuyk P.A.,Visser H.,van de Vondervoort P.J.,de Vries R.P.,Walton J.,Xiang X.,Xiong Y.,Zeng A.P.,Brandt B.W.,Cornell M.J.,van den Hondel C.A.,Visser J.,Oliver S.G.,Turner G.

The 2008 update of the Aspergillus nidulans genome annotation: a community effort.

Fungal Genet. Biol.

46

0-13

2009

686508

Roach P.L.,Schofield C.J.,Baldwin J.E.,Clifton I.J.,Hajdu J.

Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.

Protein Sci.

4

1007-1009

1995

686509

Roach P.L.,Clifton I.J.,Fueloep V.,Harlos K.,Barton G.J.,Hajdu J.,Andersson I.,Schofield C.J.,Baldwin J.E.

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.

Nature

375

700-704

1995

686510

Roach P.L.,Clifton I.J.,Hensgens C.M.,Shibata N.,Schofield C.J.,Hajdu J.,Baldwin J.E.

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.

Nature

387

827-830

1997

686511

Burzlaff N.I.,Rutledge P.J.,Clifton I.J.,Hensgens C.M.H.,Pickford M.,Adlington R.M.,Roach P.L.,Baldwin J.E.

The reaction cycle of isopenicillin N synthase observed by X-ray diffraction.

Nature

401

721-724

1999

686512

Ogle J.M.,Clifton I.J.,Rutledge P.J.,Elkins J.M.,Burzlaff N.I.,Adlington R.M.,Roach P.L.,Baldwin J.E.

Alternative oxidation by isopenicillin N synthase observed by X-ray diffraction.

Chem. Biol.

8

1231-1237

2001

686513

Elkins J.M.,Rutledge P.J.,Burzlaff N.I.,Clifton I.J.,Adlington R.M.,Roach P.L.,Baldwin J.E.

Crystallographic studies on the reaction of isopenicillin N synthase with an unsaturated substrate analogue.

Org. Biomol. Chem.

1

1455-1460

2003

686514

Long A.J.,Clifton I.J.,Roach P.L.,Baldwin J.E.,Schofield C.J.,Rutledge P.J.

Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.

Biochem. J.

372

687-693

2003

686515

Grummitt A.R.,Rutledge P.J.,Clifton I.J.,Baldwin J.E.

Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase.

Biochem. J.

382

659-666

2004

686516

Howard-Jones A.R.,Rutledge P.J.,Clifton I.J.,Adlington R.M.,Baldwin J.E.

Unique binding of a non-natural L,L,L-substrate by isopenicillin N synthase.

Biochem. Biophys. Res. Commun.

336

702-708

2005

686517

Long A.J.,Clifton I.J.,Roach P.L.,Baldwin J.E.,Rutledge P.J.,Schofield C.J.

Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine.

Biochemistry

44

6619-6628

2005

686518

Daruzzaman A.,Clifton I.J.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Unexpected oxidation of a depsipeptide substrate analogue in crystalline isopenicillin N synthase.

ChemBioChem

7

351-358

2006

686519

Howard-Jones A.R.,Elkins J.M.,Clifton I.J.,Roach P.L.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Interactions of isopenicillin N synthase with cyclopropyl-containing substrate analogues reveal new mechanistic insight.

Biochemistry

46

4755-4762

2007

686520

Stewart A.C.,Clifton I.J.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

A cyclobutanone analogue mimics penicillin in binding to isopenicillin N synthase.

ChemBioChem

8

2003-2007

2007

686521

Ge W.,Clifton I.J.,Stok J.E.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Isopenicillin N synthase mediates thiolate oxidation to sulfenate in a depsipeptide substrate analogue: implications for oxygen binding and a link to nitrile hydratase?

J. Am. Chem. Soc.

130

10096-10102

2008

686522

Ge W.,Clifton I.J.,Howard-Jones A.R.,Stok J.E.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Structural studies on the reaction of isopenicillin N synthase with a sterically demanding depsipeptide substrate analogue.

ChemBioChem

10

2025-2031

2009

686523

Ge W.,Clifton I.J.,Stok J.E.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Crystallographic studies on the binding of selectively deuterated LLD- and LLL-substrate epimers by isopenicillin N synthase.

Biochem. Biophys. Res. Commun.

398

659-664

2010

686524

Ge W.,Clifton I.J.,Stok J.E.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

The crystal structure of an LLL-configured depsipeptide substrate analogue bound to isopenicillin N synthase.

Org. Biomol. Chem.

8

122-127

2010

686525

Clifton I.J.,Ge W.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

The crystal structure of isopenicillin N synthase with delta-((L)-alpha-aminoadipoyl)-(L)-cysteinyl-(D)-methionine reveals thioether coordination to iron.

Arch. Biochem. Biophys.

516

103-107

2011

686526

Clifton I.J.,Ge W.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

Isopenicillin N synthase binds delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-thia-allo-isoleucine through both sulfur atoms.

ChemBioChem

12

1881-1885

2011

686527

Daruzzaman A.,Clifton I.J.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

The crystal structure of isopenicillin N synthase with a dipeptide substrate analogue.

Arch. Biochem. Biophys.

530

48-53

2013

686528

Daruzzaman A.,Clifton I.J.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

The interaction of isopenicillin N synthase with homologated substrate analogues delta-(L-alpha-aminoadipoyl)-L-homocysteinyl-D-Xaa characterised by protein crystallography.

ChemBioChem

14

599-606

2013

686529

Clifton I.J.,Ge W.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.

The crystal structure of an isopenicillin N synthase complex with an ethereal substrate analogue reveals water in the oxygen binding site.

FEBS Lett.

587

2705-2709

2013

686530

McNeill L.A.,Brown T.J.N.,Sami M.,Clifton I.J.,Burzlaff N.I.,Claridge T.D.W.,Adlington R.M.,Baldwin J.E.,Rutledge P.J.,Schofield C.J.

Terminally truncated isopenicillin N synthase generates a dithioester product: evidence for a thioaldehyde intermediate during catalysis and a new mode of reaction for non-Heme iron oxidases.

Chemistry

23

12815-12824

2017

686531

Rabe P.,Beale J.H.,Butryn A.,Aller P.,Dirr A.,Lang P.A.,Axford D.N.,Carr S.B.,Leissing T.M.,McDonough M.A.,Davy B.,Ebrahim A.,Orlans J.,Storm S.L.S.,Orville A.M.,Schofield C.J.,Owen R.L.

Anaerobic fixed-target serial crystallography.

IUCrJ

7

901-912

2020

686533

Rabe P.,Kamps J.J.A.G.,Sutherlin K.D.,Linyard J.D.S.,Aller P.,Pham C.C.,Makita H.,Clifton I.,McDonough M.A.,Leissing T.M.,Shutin D.,Lang P.A.,Butryn A.,Brem J.,Gul S.,Fuller F.D.,Kim I.S.,Cheah M.H.,Fransson T.,Bhowmick A.,Young I.D.,O'Riordan L.,Brewster A.S.,Pettinati I.,Doyle M.,Joti Y.,Owada S.,Tono K.,Batyuk A.,Hunter M.S.,Alonso-Mori R.,Bergmann U.,Owen R.L.,Sauter N.K.,Claridge T.D.W.,Robinson C.V.,Yachandra V.K.,Yano J.,Kern J.F.,Orville A.M.,Schofield C.J.

X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis.

Sci. Adv.

7

0-0

2021