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5.5.1.14
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generation of an OsCPS4 knockdown mutant, cps4-tos. Because Tos17 is inserted into the third intron of OsCPS4, the mature OsCPS4 mRNA is detected in the cps4-tos mutant as well as the wild type, screening Tos17 mutant lines using PCR. Mature OsCPS4 transcript levels in the cps4-tos mutant are about one sixth those in the wild-type. The cps4-tos mutant is more susceptible to rice blast fungus than the wild-type, possibly due to lower levels of momilactones and oryzalexin S in the mutant
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