EC Number   |
Protein Variants |
Reference |
|---|
    5.5.1.13 | more |
construction of chimeric proteins of Physcomitrella patens PpCPS/KS with Jungermannia subulata, the chimeric cyclases Pp131-566 /Js574-886 and Pp131-504 /Js512-886 and produce only ent-kaurene. Chimeric cyclases, Pp131-622 /Js630-886 and Pp131-714 /Js722-886, lose all enzymic activity |
714971 |
| 5.5.1.13 | more |
Jungermannia subulata JsCPS/KS peptide fragments are replaced by the corresponding Physcomitrella patens PpCPS/KS region. A PCR-amplified Jungermannia subulata JsCPS/KS DNA fragment corresponding to amino acids 574-746 is replaced by Physcomitrella patens PpCPS/KS amino acids 566-740. Four chimeric cyclases, Physcomitrella patens Pp566/Js574-746/Pp740, Pp566/Js574-721/Pp715, Pp627/Js635-721/Pp715, and Pp666/Js674-721/Pp715, have enzymic activity and produce only ent-kaurene from geranylgeranyl diphosphate, like Jungermannia subulata JsCPS/KS. The chimeric cyclase Pp566/Js574-634/Pp628 shows the same activity as wild-type PpCPS/KS, converting geranylgeranyl diphosphate to both ent-kaurene and 16alpha-hydroxy-ent-kaurane. Overview mutant chimeric constructs and enzymatic activity |
714971 |
| 5.5.1.13 | A710F |
mutant converts geranylgeranyl diphosphate to ent-kaurene as the sole product |
714971 |
| 5.5.1.13 | A710M |
mutant converts geranylgeranyl diphosphate to ent-kaurene as the sole product |
714971 |
| 5.5.1.13 | A710C |
no change in reaction product profile compared to wild-type |
714971 |
| 5.5.1.13 | A710G |
no change in reaction product profile compared to wild-type |
714971 |
| 5.5.1.13 | A710N |
no change in reaction product profile compared to wild-type |
714971 |
| 5.5.1.13 | A710S |
no change in reaction product profile compared to wild-type |
714971 |
| 5.5.1.13 | more |
sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity. Mutation of DVDD motif to AVAD leads to loss of function, mutation of DDYFD motif to AAYFD results in accumulation of ent-copalyl diphosphate |
679843 |
| 5.5.1.13 | A710F |
site-directed mutagenesis, the mutant protein shows the same enzymic activity as wild-type Jungermannia subulata JsCPS/KS |
714971 |
