EC Number   |
Protein Variants |
Reference  |
|---|
    5.4.99.39 | more |
functional expression in Saccharomyces cerevisiae mutant with disruption in oxidosqualene cyclase gene results in production of beta-amyrin |
682450 |
| 5.4.99.39 | more |
functional expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase activity results in production of beta-amyrin |
683214, 683215, 683420, 683422 |
| 5.4.99.39 | more |
construction of chimeric proteins using beta-amyrin synthase from Panax ginseng and lupeol synthase from Arabidopsis thaliana. Chimera with N-terminal half of beta-amyrin synthase and C-terminal half of lupeol synthase produces beta-amyrin and lupeol in ratio 3:1. Chimera with only the second quarter of the N-terminus from beta-amyrin synthase, produces beta-amyrin and lupeol in a 4:1 ratio, while another chimera created by mixed PCR produces beta-amyrin and lupeol in a 1:4 ratio |
683580 |
| 5.4.99.39 | C262S |
mutation in region responsible for product differentiation. Like wild-type, mutant produces beta-amyrin as sole product |
683581 |
| 5.4.99.39 | M258I/W259L |
mutation in region responsible for product differentiation. Mutant produces 40.5% beta-amyrin, 53.4% lupeol, 3.6% butyrospermol and 2.5% germanicol |
683581 |
| 5.4.99.39 | W259L |
mutation in region responsible for product differentiation. Mutant produces 30.3% beta-amyrin, 54.6% lupeol, 3.6% butyrospermol and 3.4% germanicol |
683581 |
| 5.4.99.39 | Y261H |
mutation in region responsible for product differentiation. Mutant produces 2.4% lupeol, 13.6% germanicol and 84% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol |
683581 |
| 5.4.99.39 | more |
functional expression in Pichia pastoris results in production of beta-amyrin |
683972 |
| 5.4.99.39 | more |
functional expression in Saccharomyces cerevisiae results in production of beta-amyrin |
683462, 683463, 683984, 683985 |
| 5.4.99.39 | F728A |
highly reduced activity compared to wild-type |
729601 |
