EC Number   |
Protein Variants |
Reference |
|---|
   5.1.99.5 | C181A |
no residual activity |
685319 |
| 5.1.99.5 | C181A |
no residual activity. The secondary and the tertiary structure of the mutant is not significantly affected. Residue Cys181 is responsible for L-isomer recognition and racemization. Binding thermodynamic parameters of different substrates, thermodynamic parameters of the guanidinium hydrochloride-induced denaturation |
689900 |
| 5.1.99.5 | C181S |
less than 1% of wild-type activity |
685319 |
| 5.1.99.5 | C76A |
active site mutant, crystallization data |
-, 684217 |
| 5.1.99.5 | C76A |
no residual activity |
685319 |
| 5.1.99.5 | C76A |
no residual activity. The secondary and the tertiary structure of the mutant is not significantly affected. Residue Cys76 is responsible for recognition and proton retrieval of D-isomers. Binding thermodynamic parameters of different substrates, thermodynamic parameters of the guanidinium hydrochloride-induced denaturation |
689900 |
| 5.1.99.5 | C76S |
less than 0.5% of wild-type activity |
685319 |
