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EC Number Protein Variants Commentary Reference
Show all pathways known for 5.1.2.1Display the word mapDisplay the reaction diagram Show all sequences 5.1.2.1more construction of an engineered Lactobacillus plantarum strain NCIMB 8826, which enables the production of optically pure L-lactate from raw starch for costeffective lactate production. Disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-lactate, completely abolishes D-lactate production. The DELTAldhD DELTAlarA-E mutant produces 87.0 g/l of L-lactate with an optical purity of 99.4%. A plasmid is introduced into the DELTAldhD DELTAlarA-E mutant for the secretion of alpha-amylase from Streptococcus bovis strain 148. The resulting strain produces 50.3 g/l of L-lactate from raw corn starch with a yield of 0.91 g per g of consumed sugar and an optical purity of 98.6% -, 747371
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