EC Number   |
Protein Variants |
Reference |
|---|
    4.3.99.4 | C489A |
complete loss of activity |
-, 720938 |
| 4.3.99.4 | E491A |
no activity detectable |
-, 747482 |
| 4.3.99.4 | E491Q |
no activity detectable |
-, 747482 |
| 4.3.99.4 | G821A |
complete loss of activity |
-, 720938 |
| 4.3.99.4 | more |
condtruction of a -52 amino acid truncated variant, removal of the predicted N-terminal microcompartment targeting sequence from CutC improves yields and solubility without impacting activity |
-, 728855 |
| 4.3.99.4 | T334S |
site-directed mutagenesis, the mutant retains activity |
-, 728855 |
| 4.3.99.4 | T502A |
turnover number for choline is 4400fold lower than for wild-type enzyme |
-, 747482 |
| 4.3.99.4 | T502S |
site-directed mutagenesis, the mutant retains activity |
-, 728855 |
| 4.3.99.4 | Y208F |
turnover number for choline is 215fold lower than for wild-type enzyme |
-, 747482 |
| 4.3.99.4 | Y208F/Y506F |
turnover number for choline is 1050fold lower than for wild-type enzyme |
747482 |
