EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.127 | H235P |
site-directed mutagenesis, the mutation converts Cop4 into a much more selective enzyme that produces (-)-germacrene D as the major cyclization product with 50% of total sesquiterpenes products. The mutant makes beta-ylangene, which is a diastereomer of beta-copaene and not synthesized by wild-type Cop4 |
713839 |
| 4.2.3.127 | K233I |
site-directed mutagenesis, the mutant shows a highly altered product profile compared to the wild-type enzyme with decrease in beta-copaene amounts. Cop4 loop mutant K2331 also becomes more selective for ()-germacrene D under acidic or basic reaction conditions, although less so than the wild-type enzyme |
713839 |
| 4.2.3.127 | N238L |
site-directed mutagenesis, the mutant shows a slightly altered product profile compared to the wild-type enzyme with increase in beta-copaene amounts |
713839 |
| 4.2.3.127 | N239L |
site-directed mutagenesis, the mutation converts Cop4 into a much more selective enzyme that produces (-)-germacrene D as the major cyclization product with 50% of total sesquiterpenes products. The mutant makes beta-ylangene, which is a diastereomer of beta-copaene and not synthesized by wild-type Cop4 |
713839 |
| 4.2.3.127 | T236L |
site-directed mutagenesis, the mutant shows a slightly altered product profile compared to the wild-type enzyme with increase in beta-copaene amounts |
713839 |
