EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.51 | more |
alterations of Asp76, Glu89, His115, and Arg236 do not cause significant changes in the kinetics properties |
-, 649516 |
| 4.2.1.51 | E64D |
constructed by PCR-based random mutagenesis and complementation analysis, 15% reduced activity compared to the wild-type enzyme, 4.5% increased Km, 1.7fold increased kcat |
649516 |
| 4.2.1.51 | S99M |
constructed by PCR-based random mutagenesis and complementation analysis, 30% reduced activity compared to the wild-type enzyme, insensitive to inhibition by phenylalanine |
-, 649516 |
| 4.2.1.51 | R184L |
constructed by PCR-based random mutagenesis and complementation analysis, 50% reduced activity compared to the wild-type enzyme |
649516 |
| 4.2.1.51 | F185Y |
constructed by PCR-based random mutagenesis and complementation analysis, 96% reduced activity compared to the wild-type enzyme, 26% increased Km |
649516 |
| 4.2.1.51 | T183S |
constructed by PCR-based random mutagenesis and complementation analysis, highly reduced activity |
649516 |
| 4.2.1.51 | E64Q |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
| 4.2.1.51 | E64S |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
| 4.2.1.51 | E64V |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
| 4.2.1.51 | F185L |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
