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4.2.1.161
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construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type
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